Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M...

the effect of membrane molecular weight cut off (mwco) at three levels (10, 20 & 50 kd) on dynamic behavior of permeate flux (jp), hydraulic resistances (total hydraulic resistance, rt; reversible fouling resistance, rrf; irreversible fouling resistance, rif and membrane hydraulic resistance, rm) and milk solutes rejection (protein, rp; fat, rf; lactose, rl; minerals, rm and total solids, r...

BACKGROUND A recent study suggests that patients with persistent occlusion of the middle cerebral artery (MCA) following treatment with recombinant tissue plasminogen activator (rt-PA) have better outcomes than patients with MCA occlusion not receiving rt-PA. We performed a study to elucidate possible mechanisms of this finding in a new model of thromboembolic stroke closely mimicking human pat...

Antitumor and radiosensitizing effects of (E)-2'-deoxy-2'-(fluoromethylene) cytidine (FMdC), a novel inhibitor of ribonucleotide reductase, were evaluated on nude mice bearing s.c. human C33-A cervix cancer and U-87 MG glioblastoma xenografts. FMdC given once daily has a dose-dependent antitumor effect. The maximum tolerated dose in the mice was reached with 10 daily i.p. administrations of 10 ...

introduction: in order to determine the multidrug resistance (mdr) phenotype due to p-glycoprotein expression in haematological malignancies including acute myeloblastic leukemia (aml), a well-characterized p-gp expressing cell line was required to validate and standardize flow cytometric assays and to calibrate instruments. therefore, this resistant subline of k562 was established for the firs...

AIM To report long term oncologic outcomes after adjuvant radiotherapy (RT) for stage I seminoma. METHOD We reviewed the complete data set for all patients treated at our institute between 1988 and 2005 for stage I seminoma with adjuvant RT after radical orchiectomy . RESULTS A total of 85 patients were included. The median follow-up was 15 years. The 20-year overall survival (OS) and relap...

Ratoon rice is resource efficient to produce economically sound and environmentally sustainable production. However, there no more information on the relative impact of soil tillage practices physical chemical properties, microbial biomass, enzymatic activity, grain yield in a rice–ratoon cropping system remains be tested. Systematic study under ratooning model problem solved development model....

To investigate the effects of storage temperature on the responsiveness to agonists of human platelets prepared from stored blood, we measured the aggregability and acid-base status of platelets from 96 healthy subjects before and after storage of whole blood at 4 degrees C and room temperature (RT) up to 48 hr. After 24 hr storage at 4 degrees C, there were no significant differences in agonis...

Chronic myeloid leukemia (CML) is characterized by BCR-ABL translocation and an increased number and migration of immature myeloid cells into the peripheral blood. The detection limit of the BCR-ABL transcript, particularly after treatment, is controversial. In the present study, we used quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) to monitor BCR-ABL expressi...

PURPOSE Allergen extracts from the house dust mite (HDM, Dermatophagoides pteronyssinus) are widely utilized for diagnosis and treatment of allergic diseases. It is known that allergen extracts degrade and lose potency when stored over time. METHODS This study aimed to determine the optimal conditions for stability of allergen extracts. This study was undertaken to investigate the optimal sto...