Sequential IgE-binding epitopes were identified on the molecular surface of Pis v 1 (2S albumin), 2 (11S globulin/legumin) and 3 (7S globulin/vicilin)—major allergens from pistachio (Pistacia vera) seeds—using Spot technique. They essentially consist hydrophilic electropositively charged residues well exposed allergens. Most epitopic regions do not coincide with putative N-glycosylation sites t...

three-dimensional (3D) in vitro living organs that can mimic organ and tissue structure and function in vivo will be of great benefit for a variety of biological applications from basic biology to toxicity testing and drug discovery. There have been several attempts to generate 3D tissue models but most of these models require costly equipment, and the most serious disadvantage in them is that ...