In this study, we investigated a method for the non-destructive detection of cracks based on an ultrasonic transit timing method. This method is considered to be an effective means for crack monitoring over an extensive coverage area. Aluminum specimens were prepared with a range of crack sizes and subjected to a tone burst wave to investigate the relationship between crack depth and the transi...

•Protease protection coupled APEX2 proximity proteomics of autophagosomes•Identification selective autophagy receptor-dependent cargo•Ubl conjugation and protein aggregation only modestly drive cargo engulfment•The aggrephagy receptor TOLLIP is implicated in endosomal microautophagy Autophagy deficiency fed conditions leads to the formation inclusions highlighting contribution this lysosomal de...

Miscanthus is a perennial forage plant with great potential for high stress tolerance and biomass yield. It has strong adaptability growing in saline land avoids competition grain crops arable lands. However, little known about the underlying genetic basis of adaptation to salt stress. Two diploid species genus Miscanthus, sinensis sacchariflorus, were focus this study. The transcriptome variat...

The traffic AAA-ATPase PilF is essential for pilus biogenesis and natural transformation of Thermus thermophilus HB27. Recently, we showed that PilF forms hexameric complexes containing six zinc atoms coordinated by conserved tetracysteine motifs. Here we report that zinc binding is essential for complex stability. However, zinc binding is neither required for pilus biogenesis nor natural trans...