Sucrose isomerase (SI) genes from Pantoea dispersa UQ68J, Klebsiella planticola UQ14S, and Erwinia rhapontici WAC2928 were cloned and expressed in Escherichia coli. The predicted products of the UQ14S and WAC2928 genes were similar to known SIs. The UQ68J SI differed substantially, and it showed the highest isomaltulose-producing efficiency in E. coli cells. The purified recombinant WAC2928 SI ...

Immunoglobulin haplotypes are highly polymorphic and are useful for analyses of both macro- and microdifferentiation of populations. The origins of this diversity are not known, but recent reports suggest strong selection at this locus. Increased rates of first-trimester spontaneous abortions have been reported when parents share GM phenotypes. Reduced fertility has been observed in mixed Europ...

Current Opinion in Allergy and Clinical Immunology 2008, 8:110–114 Purpose of review This review examines the relationship between exposure to workplace allergens and the risk of developing occupational allergy. Recent findings Evidence suggests that the risk of developing occupational allergy increases with allergen exposure; however, with some occupational allergens, this exposure–response re...

Of the total one-way chloride fluxes (approximately 1.4 meq/liter cell water X min) in steady state human polymorphonuclear leukocytes bathed in 148 mM Cl media, approximately 70% behaves as self-exchange mediated by a nonselective anion carrier that is not inhibited by stilbene disulfonates. Five properties of this carrier-mediated exchange were investigated: substrate saturation is seen with ...

[1] Subduction zone plate boundary megathrust faults accommodate relative plate motions with spatially varying sliding behavior. The 2004 Sumatra-Andaman (Mw 9.2), 2010 Chile (Mw 8.8), and 2011 Tohoku (Mw 9.0) great earthquakes had similar depth variations in seismic wave radiation across their wide rupture zones – coherent teleseismic short-period radiation preferentially emanated from the dee...

PURPOSE To determine how cycling with a variable (triathlon-specific) power distribution affects subsequent running performance and quantify relationships between an individual cycling power profile and running ability after cycling. METHODS Twelve well-trained male triathletes (VO2peak 4.9 ± 0.5 L/min; mass 73.5 ± 7.7 kg; mean ± SD) undertook a cycle VO2peak and maximal aerobic power (MAP) t...

Strenuous exercise leads to an increase in metabolic rate, increased production of reactive oxygen species, and compromised antioxidant defense systems. To study the effects of oxidative stress during strenuous exercise, a homogeneous group of 31 male subjects participated in a 6-month, 5 days/week training schedule involving two extreme marches of 50 km and 80 km at sea level, separated by 2 w...

[1] Ocean bottom pressure estimates from Gravity Recovery and Climate Experiment (GRACE) have been validated by comparisons with an array of in situ bottom pressure measurements. The 600 km by 600 km array comprised 46 bottom pressure sensors that were part of the Kuroshio Extension System Study (KESS). Validations in other ocean regions have been limited by available data to pointwise bottom p...