Dihydropyrimidine dehydrogenase deficiency is a major cause of severe fluoropyrimidine-induced toxicity and could lead to interruption chemotherapy or life-threatening adverse reactions. This study aimed characterize the DPYD exon sequence, mRNA expression in vivo DPD activity by plasma uracil concentration. It was carried out two groups patients with extreme phenotypes (toxicity versus control...

a series of 2-methylthio-1,4-dihydropyrimidine derivatives (ii) were synthesized in good yields by alkylation of 1,2,3,4-tetrahydropyrimidines (i) with methyl iodide in the presence of pyridine. their structures were confirmed by elemental analysis, ir and 1h nmr spectra. the compounds were tested for analgesic activity by acetic acid induced writhing method. compounds iih, iie, iik and iil sho...

Although dihydropyrimidine dehydrogenase has been purified to varying degrees from several species, very little is known about the human enzyme. The importance of this enzyme has recently been shown with cancer chemotherapy, particularly in patients with genetic deficiency of this enzyme. In the present study, this enzyme was purified 7800-fold to homogeneity from human liver by introducing sev...