Nitric oxide (NO) exerts direct effects on nephron transport. We determined the effect of NO on Na(+)-K(+)-2Cl(-) cotransport in a cell line (MMDD1) with properties of macula densa. Na(+)-K(+)-2Cl(-) cotransport was measured as bumetanide-sensitive (86)Rb(+) uptake in the presence of ouabain. MMDD1 cells expressed mRNA for the neuronal isoform of nitric oxide synthase, as well as NKCC1 and NKCC...

18and 1 lp-Hydroxylation of deoxycorticosterone and side chain cleavage of cholesterol were studied in mitochondria and submitochondrial reconstituted systems prepared from rat and bovine adrenals. A mass fragmentographic technique was used that allows determination of hydroxylation of both exogenous and endogenous cholesterol. The following results were obtained. (1) Treatment of rats with exc...

In this study, an extract of Pterocarpus marsupium Roxb. containing pterostilbene has been evaluated for its PGE2-inhibitory activity in LPS-stimulated PBMC. In addition, the COX-1/2 selective inhibitory activity of P. marsupium (PM) extract was investigated. Biological activity, as well as safety of PM extract was evaluated in healthy human volunteers. PM extract, pterostilbene and resveratrol...

Hydrogen peroxide, a relatively stable reactive oxygen species, is known to elicit vasodilation, but its underlying mechanism remains elusive. Here, we examined the role of endothelial nitric oxide (NO), prostaglandin, cytochrome P-450-derived metabolites, and smooth muscle potassium channels in coronary arteriolar dilation to abluminal H2O2. Pig subepicardial coronary arterioles (50-100 microm...

We examined the hemodynamic and tubular transport mechanisms by which platelet-activating factor (PAF) regulates salt and water excretion. In anesthetized, renally denervated male Wistar rats, with raised systemic blood pressure and renal arterial blood pressure maintained at normal levels, intrarenal PAF infusion at 2.5 ng. min(-1) x kg(-1) resulted in a small fall in systemic blood pressure (...

We solve the matrix equation XA+AX = 0, where A ∈ C is an arbitrary given square matrix, and we compute the dimension of its solution space. This dimension coincides with the codimension of the tangent space of the congruence orbit of A. Hence, we also obtain the (real) dimension of congruence orbits in C. As an application, we determine the generic canonical structure for congruence in C and a...

With most cardiovascular disease risk factors, endothelium-dependent dilation of skeletal muscle resistance arterioles is compromised, although with hypercholesterolemia, impairments to reactivity are not consistently observed. Using apolipoprotein E (ApoE) and low-density lipoprotein receptor (LDLR) gene deletion male mouse models of hypercholesterolemia at 20 wk of age, we tested the hypothes...

in the present study the distribution of rubella hemagglutination inhibition (hi) antibody titer in the sera of 1486 women of 18 to 25 years old residing in tehran were examined. sera were pretreated with acid washed kaolin and pigeon red blood cell. titers below 1:10 and in some studies below 1:20 are considered nonimmune to rubella. our results show that 10, 29% of studied population had hi a...

5 % respectively of the total (whole homogenate) activity; the postmitochondrial (1OOOOg) supernatant contained the whole of the activity. Further, on resuspending the microsomal fraction in the cell sap, all the dimethylnitrosamine demethylase activity was restored. The kinetics of dimethylnitrosamine demethylase were investigated in hepatic 1OOOOg supernatants over a substrate concentration r...

Bittern is a by-product of the manufacture salt which has high MgCl2 content. can produce Mg(OH)2 one important precursors MgO. MgO compounds be used as an antibacterial against E. coli that contaminates food. This research aims to synthesize nanoparticles well testing E.coli. Synthesis was performed using wet precipitation method with bittern reaction and technical NaOH volume ratio 1: 1 then ...