Eucalyptus species constitutes the most widely planted hardwood trees in temperate and subtropical regions. In this study, we compared the transcript levels of genes involved in lignocellulose formation such as cellulose, hemicellulose and lignin biosynthesis in two selected 3-year old hybrid Eucalyptus (Eucalyptus urophylla × Eucalyptus grandis) genotypes (AM063 and AM380) that have different ...

Neocarzinostatin (NCS) is the archetypal member of the chromoprotein family of antitumor antibiotics that consists of an apoprotein and an enediyne chromophore.1 The NCS chromophore (1) is composed of a nine-membered enediyne core, a deoxyaminosugar, and a naphthoic acid moiety (Figure 1). We have previously cloned and sequenced the NCS biosynthetic gene cluster from Streptomyces carzinostaticu...

Cell-free extracts of Ruta graveolens cell sus­ pension cultures catalyze the condensation of N-methylanthranilic acid and malonyl-CoA in the presence of ATP and Mg2+ [1,2]. After addition of CoA-SH to the incubation mixture an inhibitory effect on alkaloid formation was observed as was previously described for the chalcone synthase reaction [3, 4]. It may be assumed that the incorpo­ ration of...

Two autotrophic carbon fixation cycles have been identified in Crenarchaeota. The dicarboxylate/4-hydroxybutyrate cycle functions in anaerobic or microaerobic autotrophic members of the Thermoproteales and Desulfurococcales. The 3-hydroxypropionate/4-hydroxybutyrate cycle occurs in aerobic autotrophic Sulfolobales; a similar cycle may operate in autotrophic aerobic marine Crenarchaeota. Both cy...

The acyl adenylates participate in a variety of synthetase reactions, and their recent isolation from reactions containing substrate quantities of enzyme suggests that these compounds act as enzyme-bound intermediates (2-7). However, such evidence does not esclude the possibility, emphasized by Boyer, Mills, and Fromm, that the adenylate occurs only as a free side product that is in equilibrium...

The mbd cluster encoding genes of the 3-methylbenzoyl-CoA pathway involved in the anaerobic catabolism of 3-methylbenzoate and m-xylene was characterized for the first time in the denitrifying β-Proteobacterium Azoarcus sp. CIB. The mbdA gene product was identified as a 3-methylbenzoate-CoA ligase required for 3-methylbenzoate activation; its substrate spectrum was unique in activating all thre...

The phenylpropanoid pathway is a major secondary metabolite that helps plants overcome biotic and abiotic stress produces various byproducts promote human health. Its byproduct caffeoylquinic acid soluble phenolic compound present in many angiosperms. Hydroxycinnamate-CoA shikimate/quinate transferase significant enzyme plays role accumulating CQA biosynthesis. This study analyzed transcriptome...

Anaerobic benzoate degradation by the phototrophic bacterium Rhodopseudomonas palustris has been proposed to proceed via aromatic ring reduction reactions leading to cyclohex-1-ene-1-carboxyl-coenzyme A (CoA) formation. The alicyclic product is then proposed to undergo three beta-oxidation-like modifications resulting in ring cleavage. Illuminated suspensions of benzoate-grown cells converted [...