BACKGROUND This study examined whether activation of innate immunity and alterations of carbohydrate and lipid metabolism precede development of subclinical mastitis (SCM). METHODS Blood samples were collected from the coccygeal vein from 100 Holstein dairy cows at -8, -4, disease diagnosis week, and +4 weeks postpartum. Six healthy cows (controls - CON) and six cows that showed clinical sign...

BACKGROUND The objective was to evaluate if cortisol concentrations are associated with the resumption of luteal activity in postpartum, primiparous cows exposed to bulls. The hypotheses were that 1) interval from start of exposure to resumption of luteal activity; 2) proportions of cows that resumed luteal function during the exposure period; and 3) cortisol concentrations do not differ among ...

The present experiment was undertaken to verify if the demand of tissues for pteroylglutamic (folic) acid, evaluated by serum clearance and urinary excretion of folates, is different between multiparous dairy cows in late gestation (five gestating dry cows, 52.6 (SD 8.4) d before parturition), and in early lactation (four lactating non-gestating cows, 18.0 (SD 5.9) d after parturition). On day ...

In dairy cows the peripartum period involves endocrine and metabolic changes to compensate for the increased energy requirement aggravated by reduced feed intake. Cows adjust to the resulting negative energy balance with the mobilization of lipids from adipose tissue that, if excessive, could lead to many transition disorders compromising the offspring’s growth and well-being. The aim of this s...

The objective of this experiment was to evaluate the effects of glucose infusion on serum concentrations of glucose, insulin, and progesterone (P4), as well as mRNA expression of hepatic CYP2C19 and CYP3A4 in nonlactating, ovariectomized cows in adequate nutritional status. Eight Gir × Holstein cows were maintained on a low-quality Brachiaria brizantha pasture with reduced forage availability, ...

The present study was conducted at Livestock Research Station, Palamaner, Chittoor district, Andhra Pradesh to estimate female reproductive hormone values in Punganur cattle. The blood samples were collected from the twelve Punganur cows on 0, 3, 6, 9, 12, 15, 18 and 21 days of estrous cycle. The serum was separated from the blood samples and was transferred into sterile eppendorf tubes, labele...

BACKGROUND: Leptospirosis is a worldwide zoonosis causedby Leptospira interrogans. Leptospirosis results in decreasedmilk production, abortion, stillbirth, infertility and mortality,which causes financial loss in the cattle industry. OBJECTIVES:The aim of this research was to perform a serological andbacteriological study of leptospirosis in 6 industrial dairy herdsand 3 feedlots with previous ...

The purpose of this study was to assess the diagnostic value of fibrinogen, haptoglobin, and serum amyloid A determination in the monitoring of the treatment of limb diseases in dairy cows. Fourteen lame cows were examined, while 10 clinically healthy cows constituted the control group. Blood samples from the ill animals were collected on three occasions: (1) upon arrival at the clinic, (2) bet...

Six apparently healthy, non-lactating and non-pregnant Sahiwal cows and Murrah buffalo each above two years of age were selected to evaluate the effect of seasonal variations on hematological parameters. Blood samples were collected aseptically by jugular vein puncture during peak winter and peak summer seasons. The present investigation revealed the mean values of Hb, PCV, TEC, neutrophils, MC...

Milk samples were taken daily or twice weekly, and blood samples twice weekly, from six clinically healthy dairy cows. Acetone concentration was determined by a new headspace gas-chromatographic method that proved to be suitable in terms of practically, sensitivity and precision. The concentration of acetone in milk was closely correlated with that in blood (r2 = 0.967). There was no relationsh...