A new fluorogenic substrate for serine proteinases, bis(N-benzyloxycarbonyl-L-argininamido)Rhodamine [(Cbz-Arg-NH)2-Rhodamine], was synthesized, purified and chemically and enzymically characterized. This compound, which employs Rhodamine as a fluorophoric leaving group, is the first in a series of substrates designed to measure the amidase activity of proteinases. Cleavage of one of the amide ...

The expression of high levels of P-glycoprotein (Pgp) in circulating mononuclear cells allowed us to use an ex vivo assay as a surrogate measure of Pgp antagonism. Efflux of rhodamine from CD56(+) cells was measured before the start of PSC 833 and at varying times thereafter. Patients receiving PSC 833 had decreased rhodamine efflux from their circulating CD56(+) cells. Time course studies show...

Pgp is expressed on normal haemopoietic progenitor cells. The significance of the efflux pump in protecting normal progenitors for anthracycline toxicity is not defined and is the subject of this study. Pgp was measured in CD34+ progenitors with a rhodamine efflux assay. A high efflux, modulated by verapamil, was only found in a distinct subpopulation (20-30%). Pgp measured by the monoclonal an...

Mesoporous silica nanoparticles (MSN) have potential as drug delivery and controlled release devices due to their high surface area and absorption capabilities. The effect of surface charge and pH on the release of the fluorescent dye, rhodamine 6G, from MSN has been studied. Release profiles of rhodamine 6G from bare and amine-coated MSN at pH 5.0 and 7.4 are very different and demonstrate tha...

A novel method for the separation/analysis rhodamine B has been described. The ionic liquid (1-tetradecyl-3-methylimidazolium bromide)/anion surfactant (sodium dodecyl sulfate)/NaCl two-phase systems (ATPSs) is presented as a simple, rapid and effective sample pretreatment technique coupled with ultraviolet spectrometry for analysis rhodamine B in soft drink. The effects of parameters on the AT...

The rhodamine system is a flexible framework for building small-molecule fluorescent probes. Changing N-substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, provid...

1 Moureau N, Lamperti M, Kelly LJ, et al. Evidence-based consensus on the insertion of central venous access devices: definition of minimal requirements for training. Br J Anaesth 2013; 110: 347–56 2 Breschan C, Platzer M, Jost R, et al. Consecutive, prospective case series of a new method for ultrasound-guided supraclavicular approach to the brachiocephalic vein in children. Br J Anaesth 2011;...

110 cases of optic neuritis were prospectively studied. 62 (56%) developed multiple sclerosis, 13 were lost to survey. However, eight of these had recurrent episodes of optic neuritis. If recurrent episodes of optic neuritis are accorded the same significance as demyelination elsewhere in the CNS, then the incidence of multiple sclerosis in the series rose to 71%, similar to that predicted on a...

conclusions nx-dcp correlated positively with fibrosis stage and could discriminate well between hcv-related patients with or without significant fibrosis. moreover, nx-dcp had a similar predictive ability to the abovementioned models, and thereby could be a new noninvasive prediction tool for fibrosis. results increase in serum nx-dcp was significantly related to increase in fibrosis stage (p ...

in this research, after carrying out chemical tests measuring the moisture, protein and ash contents on rice flour according to standard methods, gluten- free breads were prepared from rice flour combined with 0.5% carboxy methyl cellulose (cmc) and 1% -κcarrageenan gum. a sample lacking gum was also produced as control. rice breads (with gum and without gum) were then produced by semi-industri...