Apparently free-base cytokinins can interact with cupric ions in a specific manner. Oxidation of NADH by a horseradish peroxidase system was strongly promoted by such cytokinins provided cupric ions were present. Oxidation was promoted by 5 micromolar kinetin, zeatin, 6-benzylaminopurine (BA), or 6-(Delta(2)-isopentenylamino)purine (2iP) but not by adenine, 6-methylaminopurine or 6,6-dimethylam...

Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability to metabolize N(6)-benzyladenine (BA). Formation of N(6)-benzyladenosine-5'-mo...

The influence of pre-sowing treatments using hydropriming, osmopriming and halopriming in laboratory and/or nursery tests on seedling emergence, seedling weight and plant growth of endive and chicory was examined. Halopriming (KNO3) or growth regulators (gibberelic acid; GA3) improved the rate of germination of endive and chicory and reduced the mean germination time needed. 30 min pre-sowing t...

A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtaine...

An efficient in vitro regeneration protocol has been developed from shoot tips of Drosera burmannii Vahl., a carnivorous plant of north-east India. Various plant growth regulators were used to study their efficacy in the induction of multiple shoots and roots. Of the various treatments, the maximum number of shoots (28.8 ± 1.5) and roots (9.7 ± 0.6) was observed in one-fourth strength standard ...

In vitro regeneration of plants from root cultures of Swertia chirata Buch.-Ham. ex Wall was obtained. Root explants from axenic shoot cultures were used for shoot induction. Optimal shoot regeneration without any callus intervention was observed on 1/2 MS (Murashige and Skoog’s medium, 1962) + 4.44 μM 6, benzylaminopurine (BAP) +1.07 μM α-naphthalene acetic acid (NAA).Regenerated shoots were f...

Protein synthesis in gibberellin-treated lettuce (Lactuca sativa) seeds has been studied during the lag phase between the beginning of imbibition and the first signs of radicle protrusion. When compared to the water-imbibed controls, both polyribosome populations and radioactive leucine incorporation into protein increase in the embryos of GA(3)- induced seeds early in the imbibition period. Si...

The purpose of this study was to evaluate the effects of different levels of salinity stress and sprayed of the 6-benzylaminopurine (benzyl adenine) on morphological and biochemical characteristics of winter jasmine. Therefore, an experiment was conducted in 2016-2017 growing seasons in research center of greenhouse production of Islamic Azad University, Isfahan (Khorasgan) Branch. The experime...

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic em...