Phlebotomus papatasi is an important vector of L. major in Iran. P. papatasi was collected from peridomestic animal shelters, inside and around the houses and also the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in several provinces in Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, which is a maternally-inherited gene marker, was used to see if there is any "isolatio...

We have shown that an automated DNA sequencer is applicable to fluorescence-based detection of fragments in DNase I footprinting. We demonstrated the potential of long-range and highly sensitive DNase I footprinting taking advantage of an infrared-fluorescence automated DNA sequencer. Footprints of human transcription factor SpI were reproducibly detected ranging approximately between 100 and 7...

background : haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.   methods: the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundr...

tomato yellow leaf curl virus (tylcv) is considered as one of the most important tomato viruses in tropical and subtropical areas. in order to identify and investigate the presence of this virus in fields , greenhouses and cultivated tomato plants in tuneles of khorasan razavi, southern and northern khorasan provinces, during 2009 and 2011, plants which have been showed the symptoms were collec...

The use of invertebrate-derived DNA (iDNA) is a promising non-invasive tool to monitor wildlife. While most studies have been carried out in dense tropical and sub-tropical forests focused on the single category invertebrates, this study compares flies mosquitoes-derived assess vertebrate diversity semi-urban environments. We conducted our sampling four different forest plots Berlin, Germany. P...

the ovine melatonin receptor 1a (mtnr1a) and aromatase (cyp19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in kurdi sheep at kurdi sheep breeding station located in shirvan, iran. the genomic dna was extracted by guanidine thiocyanate-silica gel method. polymerase chain reaction was carried out to amplify 824 bp...

Human erythrocyte glycophorin C plays a functionally important role in maintaining erythrocyte shape and regulating membrane mechanical stability. Immunochemical and serologic studies have identified a number of glycophorin C variants that include the Yus, Gerbich, and Webb phenotypes. We report here the molecular characterization of these variants. Amplification of glycophorin C mRNA from the ...

identification of effective major genes releted with energy balance, milk production, fertility, immunity, as well as feed consumption constitutes some of the most important and strategic objectives in animal breeding and genetics researches. among the major genes, leptin is one of the largely effective ones the polymorphism of which is closely related with important economic traits in livestoc...

Objective(s):  Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provi...