The isoenzymesof alkalinephosphatase(ALP; EC 3.1.3.1) present in the .sera of 116 patients with hepatobiliary disease were studied by electrophoresis on cellulose acetate. Individual liver isoenzyme bandswere identified an quantitated by densitometnc scanning. The occurrence and magnitude of increase of the isoenzyme bands of liver ALP (a2 alone or a1 and cx2) are related to diagnosis. Of the c...

Bee venom is a complex mixture of proteins (enzymes and peptides) with unique pharmacological activities. The main enzymes in bee venom are hyaluronidase and phopholiphase A2. Melittin in high concentrations also has caused hemolysis of red blood cells. This study was conducted to evaluate effects of honeybee venom on hematological characteristics of albino rats. Rats were divided in 3 groups v...

itor in blood plasma is higher than that in serum obtained from the blood clotted in the presence ofcalcium ions, but is the same as that in serum obtained in the absence of calcium ions. Radiolabeled a2-plasmin inhibitor was covalently bound to fibrin only when calcium ions were present at the time of clotting of plasma or fibrinogen. Whereas, when batroxobin, a snake venom enzyme that lacks t...

Bleomycins, a group of glycopeptide antibiotics differ from one another in their terminal amine moieties. 14C-Methyl-labeled 3-aminopropyl-dimethylsulfonium was confirmed to be incorporated into the amine moiety of bleomycin A2. Addition of amines of bleomycin A2, A2'-a, A2'-b, A2'-c, A5, B2 and B4 to fermentation media increased the rate of production of the bleomycin which contained the added...

PURPOSE The aim of this study was to demonstrate the synthesis and cellular distribution of group II phospholipase A2 and lysozyme in the main and accessory lacrimal glands. METHODS The authors studied samples of normal main lacrimal glands of seven autopsied subjects and accessory lacrimal glands of eight patients who underwent ptosis surgery. The specimens were immunostained with a rabbit a...

Background—Although adrenergic activation plays a major role in the initiation of experimental myocardial ischemia, the significance of a-adrenergic coronary constriction in humans has been questioned. The present study assessed the impact of selective a-adrenergic receptor activation in patients with normal or atherosclerotic coronary arteries. Methods and Results—In 39 patients, coronary bloo...