Background: This study aimed to compare the use of next-generation sequencing (NGS) and single-nucleotide polymorphism (SNP) array in preimplantation genetic testing for aneuploidy (PGT-A) same blastocyst. Methods: We performed a retrospective on 67 embryos (from 23 couples), where PGT-A was carried out. A trophectoderm (TE) biopsy blastocyst, 24-chromosomal ploidy status analyzed. Initially, 2...

BACKGROUND Recent advancement in both human embryology and preimplantation genetic screening has created a completely new situation for human assisted reproduction. Embryos, typically at blastocyst stage, are biopsied and screened by DNA microarray or next-generation sequencing before cryopreservation, and then euploid embryos are warmed for transfer. Increased embryo implantation rates have be...

objective: rice bran oil (rbo) is extracted from the outer layer of rice. little information is available regarding its safety. the present study was conducted to assess its safety in chicken embryo model. materials and methods: rbo was injected on day 4 of incubation of chickens. the tissues and serum samples were collected. oxidative stress parameters in the liver, kidney and brain and bioche...

Uniparental disomy (UPD) has been shown to be rare in human normal blastocysts, but its frequency in discarded morphologically abnormal embryos and its relevance to embryonic self-correction of aneuploid remains unknown. The aim of this study was to detect UPD in discarded morphologically abnormal embryos. Both discarded morphologically abnormal embryos, including zero-pronuclear zygotes (0PN),...

In recurrent pregnancy loss, current practice often fails to make a diagnosis, as the fetal causes of pregnancy loss are usually ignored and only the maternal factors are assessed. Hence, it has been almost impossible to show that any treatment has an effect. This presentation is concerned with the fetal causes of recurrent pregnancy loss. The maternal causes are well known and include: uterine...

background: in this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. methods: the developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of 20 ...

the latest micromanipulative technique, intracytoplasmic sperm injection (icsi), has been very successful in cases of severe male factor infertility. the main objective of this study is to report the results of our first experience with icsi in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (ivf). normal fertil...

Background: Regulation of gene expression in mammalian embryos is not completely known. Pre-implantation embryos need maternal RNA and proteins synthesized during oogenesis, to regulate development before mater-embryo transition, as the grown oocyte and the 1-cell zygote are transcriptionally silent. There are some oocyte-specific genes called maternal effect genes which may account for this ea...

Background GnRH agonist triggering is one of the strategies for ovulation triggering and final maturation of oocytes. So , should be notice for luteal phase support in these cycles. At the first it was began for prevention of severe OHSS but it was associated with luteal phase problem and lower pregnancy rate due to luteolysis effects of GnRH agonists. So, two other alternative strategies have ...