A coupling process of anaerobic methanogenesis and electromethanogenesis was proposed to treat high organic load rate (OLR) wastewater. During the start-up stage, acetate removal efficiency of the electric-biological reactor (R1) reached the maximization about 19 percentage points higher than that of the control anaerobic reactor without electrodes (R2), and CH4 production rate of R1 also incre...

In the present investigation, we prepared four different solvent fractions (chloroform, hexane, butanol, and ethyl acetate) of Moringa oleifera extract to evaluate its anti-inflammatory potential and cellular mechanism of action in lipopolysaccharide (LPS)-induced RAW264.7 cells. Cell cytotoxicity assay suggested that the solvent fractions were not cytotoxic to macrophages at concentrations up ...

Mutation of the ACN1 (acetate non-utilizing 1) locus of Arabidopsis results in altered acetate assimilation into gluconeogenic sugars and anapleurotic amino acids and leads to an overall depression in primary metabolite levels by approx. 50% during seedling development. Levels of acetyl-CoA were higher in acn1 compared with wild-type, which is counterintuitive to the activity of ACN1 as a perox...

For an economically competitive biological process, achieving high carbon yield of a target chemical is crucial. In biochemical production, pyruvate and acetyl-CoA are primary building blocks. When sugar is used as the sole biosynthetic substrate, acetyl-CoA is commonly generated by pyruvate decarboxylation. However, pyruvate decarboxylation during acetyl-CoA formation limits the theoretical ma...

AIn increase of C'4O., pro(luction from acetate-i-C'4 and acetate-2-C14 in corn roots by 2,4-D treatment has been reported; the percenitage increase was larger fronm the methbl carbon than from the carboxyl carbon (4). Usinig root tissues it was found that 2,4-D cause(d an increase in the amliouint of glucose catabolized via the pentose phosphate pathxway in root tissues (3). Whlen bean stem ti...

The degradation of organic matter in the anoxic seabed proceeds through a complex microbial network in which the terminal steps are dominated by oxidation with sulfate or conversion into methane and CO2 The controls on pathway and rate of the degradation process in different geochemical zones remain elusive. Radiotracer techniques were used to perform measurements of sulfate reduction, methanog...

No. Iyer367 Determination of the X-ray Crystal Structure of Phosphotransacetylase from Methanosarcina thermophila. P. Iyer, H. Yennawar (Penn State), K. Rajashankar (BNL, NSLS) and J. Ferry (Penn State) Beamline(s): X9A Introduction: Phosphotransacetylase is a key enzyme in the pathway of methanogenesis from acetate. In the archaeon Methanosarcina thermophila, phosphotransacetylase, along with ...