Acetyl-coA carboxylase isolated from freezeclamped livers of fed rats has relatively low phosphate content (6.0 mol of PJmol of subunit) and high specific activity (3.6 unitslmg in the absence of citrate). The enzyme from rats fasted for 12, 18, 24, and 48 h exhibited decreasing specific activities of 2.75, 1.86, 1.7, and 0.9 unitslmg, respectively. Citrate activated all preparations of carb...

2',3'-O-(2,4,6-Trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP) is a fluorescent analogue of ATP. MgTNP-ATP was found to be an allosteric activator of pyruvate carboxylase that exhibits competition with acetyl CoA in activating the enzyme. There is no evidence that MgTNP-ATP binds to the MgATP substrate binding site of the enzyme. At concentrations above saturating, MgATP activates bicarbona...

abstract in order to determine efficacy of some registered graminicides in control of resistant and susceptible ryegrass biotypes, two greenhouse trials were conducted at weed research department of iranian plant protection research institute during 2007. experimental design was completely randomized design with eight replications. the susceptible and resistant biotypes were treated by 19 herbi...

Biotin has a profound effect on the metabolism of rhizobia. It is reported here that the activities of the biotin-dependent enzymes acetyl-coenzyme A carboxylase (ACC; EC 6.4.1.2) and propionyl-coenzyme A carboxylase (PCC; EC 6.4.1.3) are present in all species of the five genera comprising the Rhizobiaceae which were examined. Evidence is presented that the ACC and PCC activities detectable in...

An investigation was made of the interaction of pyruvate carboxylase with its allosteric effector, acetyl-CoA, and the velocity profile of the deacylation of acetyl-CoA as a function of acetyl-CoA concentration indicated that this ligand does not bind to this enzyme in a positive homotropic co-operative manner. An examination was therefore made of the factors that contribute to the sigmoidicity...

of medium chain fatty acids (MCFAs; C 8 –C 12) requires their activation inside peroxisomes by an acyl-CoA syn-SFB Biomembrane Research Center Institut fü r Biochemie und Lebensmittelchemie thetase, Faa2p. Myristoylation of membrane-associated proteins is catalyzed by Nmt1p (for review on lipid syn-Technische Universitä t Graz Petersgasse 12 thesis in yeast, see Kohlwein et al., 1996). S. cerev...

BACKGROUND Hypothalamic AMPK acts as a cell energy sensor and can modulate food intake, glucose homeostasis, and fatty acid biosynthesis. Intrahypothalamic fatty acid injection is known to suppress liver glucose production, mainly by activation of hypothalamic ATP-sensitive potassium (K(ATP)) channels. Since all models employed seem to involve malonyl-CoA biosynthesis, we hypothesized that acet...

Treatment of rat epididymal adipose tissue with insulin for a few minutes leads to a substantial increase in the rate of fatty acid synthesis, which is explained in part by rapid activation of the cytoplasmic enzyme acetyl-CoA carboxylase (Denton, 1975; Denton et al., 1977; Brownsey & Denton, 1979). The effects of insulin on fatty acid synthesis are opposed by adrenaline, but the changes in kin...

Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction, especially under harsh operating conditions. The rich metabolic versatility of this species, however, comes at the price limited formation acetyl-coenzyme A (CoA) from sugar substrates. Since acetyl-CoA a key precursor number added-value products, in work we deployed an silico-guided rewiring program central ...

The abnormal accumulation of lipids due to myo-inositol deficiency in Saccharomyces carlsbergensis, and the mechanism involved was investigated. The deficient cells contained much more neutral lipids with a greater ratio of unsaturated fatty acids compared to the supplemented cells, whereas there was no significant change in their phospholipid contents. The biosynthesis of fatty acids and stero...