ABSTRACT: The interaction of two cationic dyes namely, acridine orange and pinacyanol chloride with an anionic polyelectrolyte, heparin, has been investigated by spectrophotometric method.The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter wavelengths. The stability of the complexes formed between acridine orange and heparin w...

The aim of the present study was to verify whether culturing testicular tissue, to obtain a higher percentage of motile spermatozoa and a better post-thaw recovery rate, affected the ratio between single/double-stranded sperm DNA and, consequently, DNA sensitivity to damage. Testicular biopsy samples from men with obstructive and secretory azoospermia, candidates for assisted reproductive treat...

purpose: to image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective dna stain, in rats methods: corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 long-evans rats. one week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...

Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...

Most laboratory methods used to evaluate semen quality have not correlated highly with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probes has enabled a more widespread analysis of sperm attributes, and in conjunction with the flow cytometer, permit the evaluation of a large number of spermatozoa. A number of characteristics of sperm ...

The neutrophil count, immature:total neutrophil ratio, C reactive protein assay, nitroblue tetrazolium test and an acridine orange leucocyte cytospin test were evaluated for the diagnosis of neonatal bacteraemia. The acridine orange leucocyte cytospin test gave the highest specificity and positive predictive accuracy, but was less sensitive than the neutrophil count, C reactive protein assay or...

BACKGROUND The aim of this study was to determine if only sperm with double stranded DNA could bind to the human zona pellucida (ZP). METHODS Sperm samples from 124 infertile men with a range of semen abnormalities were studied. Oocytes that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile s...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We are delighted to read the insightful commentary by Dr. Evenson (1), a well-known pioneer in the field of SDF testing, in response to the practice recommendations on clinical utility of sperm DNA fragmentation (SDF) testing by Agarwal et al. (2). The process of protamination during spermiogenesis was discovered as e...

introduction: during spermiogendsis, sperm chromatin histones are replaced by protamine. after fertilization, the sprem nucleus is decondenced by glutathione (gsh). in this survey, we study the effect of various concentrations of gsh on human in vitro sperm decondensation. material and methods: after preparation and analysis of semen samples, sperm pellet was washed by ham's f-10 medium twice a...