We have cloned and sequenced a 2845 bp cDNA representing the 3'-end of either a new picorna-like virus species or genotype of Solenopsis invicta virus-1 (SINV-1). Analysis of the nucleotide sequence revealed 1 large open reading frame. The amino acid sequence of the translated open reading frame was most identical to structural proteins of SINV-1 (97%), followed by the Kashmir bee virus (KBV, 3...

The diversity of eukaryote organisms and viruses associated with wild bees remains poorly characterized in contrast to the well-documented pathosphere of the western honey bee, Apis mellifera. Using a deliberate RNA shotgun metagenomic sequencing strategy in combination with a dedicated bioinformatics workflow, we identified the (micro-)organisms and viruses associated with two bumble bee hosts...

The Guillain Barre Syndrome (GBS) is an acute immune mediated progressive polyneuropathy having monophasic illness leading to paralysis. clinical features are ascending symmetrical muscle weakness that may lead respiratory failure. Diagnosis based upon presentation and supported by a lumbar puncture with CSF analysis demonstrating albumin cytological dissociation electrophysiological studies. O...

West Nile virus (WNV) is one of the leading causes of insect-borne encephalitis and acute flaccid paralysis in the US. Acute flaccid paralysis is a potentially serious illness, which manifests itself as a Guillain-Barré-like syndrome with generalized weakness and shortness of breath. We report a case involving a patient who presented with acute flaccid paralysis due to WNV infection and was suc...

Acute Flaccid Myelitis (AFM), a polio-like paralysis in children no older than 21, has recently reemerged and increasing numbers of such cases have been reported worldwide since 2012. Accurate and early diagnosis of this condition could help with better management of the disease. A 9- year- old girl with chief complaint of headache, fever and vomiting was subsequently affected by an acute...

Acute weakness associated with West Nile virus (WNV) infection has previously been attributed to a peripheral demyelinating process (Guillain-Barré syndrome); however, the exact etiology of this acute flaccid paralysis has not been systematically assessed. To thoroughly describe the clinical, laboratory, and electrodiagnostic features of this paralysis syndrome, we evaluated acute flaccid paral...

Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infec...

Viruses are one of the most potential risk factors that negatively affect different life stages honey bees. This study was conducted to determine presence infections caused by deformed wing virus (DWV), black queen cell (BQCV), and acute bee paralysis (ABPV) in bees beekeeping regions Burdur, along with obtaining information about their prevalence this particular region. In our study, adult wer...

Mexico is a major honey producer, but not much information exists about the health status of bees (Apis mellifera L.) in country. This study was conducted to determine sanitary adult Mexico’s five beekeeping regions. Samples from 369 apiaries were diagnosed identify pathogens such as Varroa destructor, which quantified, Acarapis woodi, Nosema spp., and viruses. Colonies also inspected for prese...