A series of adenosine deaminase (ADA) retroviral vectors were designed and constructed with the goal of improved performance over the PA317/LASN vector currently used in clinical trials. First, the bacterial selectable-marker neomycin phosphotransferase (neo) gene was removed to create a "simplified" vector. Second, the Moloney murine leukemia virus long terminal repeat (LTR) promoter used for ...

A marked tissue-specific increase in erythrocyte adenosine deaminase (ADA) activity is associated with an autosomal dominantly inherited hemolytic anemia. We investigated the molecular basis of ADA overproduction by studying reticulocyte ADA mRNA from affected individuals. Analysis of proband reticulocyte ADA cDNA clones revealed normal sequence. RNase mapping demonstrated that the amount of AD...

One major difficulty that faces developers of concurrent and distributed software is analysis for concurrency based faults like deadlocks. Petri nets are used extensively in the verification of correctness of concurrent programs. ECATNets are a category of algebraic Petri nets based on a sound combination of algebraic abstract types and high-level Petri nets. ECATNets have 'sound' and 'complete...

Ada has been successfully used in many dependable real−time applications, which have undoubtedly benefit of its major strengths: The well defined language semantics [1], the strong type checking, structuring mechanisms like packages and not to forget the Ada Semantic Interface Standard [2] supporting the development of code analysis, verification and testing tools. But an Ada system can not be ...

This paper presents a brief description of the current work on a tool that analyses temporal behaviour of Ada/RavenSPARK programs. The approach takes as a basis two previous publications that introduce innovative methods in the field of verification of realtime systems. The development of a tool that automatically generates models (timed automata) from Ada/RavenSPARK source code and uses the Up...

Adenosine deaminase (ADA) deficiency causes an autosomal recessive form of severe combined immunodeficiency and also less severe phenotypes, depending to a large degree on genotype. In general, ADA activity in cells of carriers is approximately half-normal. Unexpectedly, healthy first-degree relatives of two unrelated ADA-deficient severe combined immunodeficient patients (mother and brother in...

Intention der Fachgruppe: Software garantiert häufig direkt die Sicherheit für Menschen. Ada wird in internationalen Sicherheitsstandards empfohlen und unterstützt in einzigartiger Weise moderne Analyse, Design und Programmiermethoden und damit die Entwicklung großer zuverlässiger Software-Systeme. Die Fachgruppe will über die Konzepte von Ada und deren Bedeutung für die Softwaretechnologie inf...

The adaptive response is a cellular process to induce DNA repair enzymes in response to a challenge of alkylating agents. In this process Ada protein, the product of the ada gene, plays a major role; it accepts the methyl groups of the methylated DNA at the cysteine residues of its own molecule, and the methylated form of Ada protein promotes transcription of its own gene, thereby triggering in...