The appearance of P-adrenergic receptors and isoproterenol-stimulated adenylate cyclase during differentiation of embryonic quail muscle cells in tissue culture has been examined. P-Adrenergic receptors first appear during the fusion stage of myogenesis. ['"I]]Iodohydroxybenzylpindolol (['2sI]IHYP) was used as the ligand to characterize &adrenergic receptors. In equilibrium binding studies, mat...

Background—Autoantibodies against synthetic peptides of b-adrenergic receptors have been observed in human cardiomyopathy. However, it has never been shown that such antibodies really interact with native human b-adrenergic receptors, nor has the clinical impact of such an interaction been investigated in larger groups of patients. Methods and Results—We screened 104 patients with dilated or is...

Adrenergic receptors for adrenaline and noradrenaline belong to the large multigenic family of receptors coupled to GTP-binding proteins. Three pharmacologic types have been identified: alpha 1-, alpha 2-, and beta-adrenergic receptors. Each of these has three subtypes, characterized by both structural and functional differences. The alpha 2 and beta receptors are coupled negatively and positiv...

BACKGROUND We have recently shown that genetic inactivation of phosphoinositide 3-kinase gamma (PI3Kgamma), the isoform linked to G-protein-coupled receptors, results in increased cardiac contractility with no effect on basal cell size. Signaling via the G-protein-coupled beta-adrenergic receptors has been implicated in cardiac hypertrophy and heart failure, suggesting that PI3Kgamma might play...

The various subtypes of receptors for catecholamines, termed adrenergic receptors, represent excellent models for the study of receptor-mediated transmembrane signalling because of their ubiquity, their coupling to well-defined effector mechanisms, and the clinical importance of drugs which interact with them. In general, the adrenergic receptor systems consist of three components which are cou...

It is well known that the renal nerve plays an important role in the regulation of renal functions such as sodium and water reabsorption in the tubules as well as in the pathogenesis of essential hypertension. The renal sympathetic nerves innervate to the basement membranes of almost all nephron segments. Specific adrenergic receptors and intracellular signal transduction systems are located in...

Background—We have recently shown that genetic inactivation of phosphoinositide 3-kinase (PI3K ), the isoform linked to G-protein–coupled receptors, results in increased cardiac contractility with no effect on basal cell size. Signaling via the G-protein–coupled -adrenergic receptors has been implicated in cardiac hypertrophy and heart failure, suggesting that PI3K might play a role in the path...

Sympathetic nerves may play a role in vascular disorders of the eye. In the present study, we hypothesized that activation of beta3-adrenergic receptors on retinal endothelial cells would promote migration and proliferation of these cells, two markers of an angiogenic phenotype. We show, for the first time, expression of beta3-adrenergic receptors on cultured retinal endothelial cells. Activati...

In this study the effect of adrenergic receptor agonists and antagonists on physostigmine induced yawning was investigated. Intraperitoneal injection of different doses of physostigmine (0.03, 0.05, 0.1 and 0.2 mg/kg) caused yawning in white rats. The greatest response was seen at a dose of 0.2 mg/kg physostigmine. Phenylephrine, an α1 agonist, and clonidine, an α2 agonist, led to a decrease in...

ATP, norepinephrine and NPY are co-released by sympathetic nerves innervating arteries. ATP elicits vasoconstriction via activation of smooth muscle P2X receptors. The functional interaction between neuropeptide Y (NPY) receptors in arteries is not known. In this study we investigate the effect on P2X1-dependent mouse mesenteric Suramin or P2X1 antagonist NF449 abolished α,β-meATP evoked vasoco...