A model system to study the involvement of cAMP-mediated regulation of a cellular process such as hyphal morphogenesis was investigated. Impairment of polarized growth was observed when Mucor rouxii sporangiospores were grown in the presence of N(6)-cAMP analogues and of SQ 65,442, a cAMP phosphodiesterase inhibitor. Together with an effect on isodiametric growth, there was increased pigmentati...

pletely or largely depressed by oxygen lack, respiratory inhibitors and uncouplers of oxidative phosphorylation when the incubation medium was devoid of glucose (1-7). Similar inhibition has been found of compound 48/80-induced histamine release from rat lung tissue and of degranulation of rat mesentery mast cells (6, 8, 9). Since these inhibitions were largely overcome by the presence of gluco...

The respiratory chain of E. coli is branched to allow the cells' flexibility to deal with changing environmental conditions. It consists of the NADH:ubiquinone oxidoreductases NADH dehydrogenase I and II, as well as of three terminal oxidases. They differ with respect to energetic efficiency (proton translocation) and their affinity to the different quinone/quinol species and oxygen. In order t...

PURPOSE To describe the conjunctival bacterial spectrum of our patients undergoing intraocular surgery and their antibiotic sensitivity during the study period. METHODS A retrospective study of preoperative conjunctival culture of patients consecutively scheduled for intraocular surgery from 21 February 2011 to 1 April 2013. Specimens were directly seeded onto blood-agar and MacConkey-agar (a...

26 27 Two bacterial species, Pseudomonas aeruginosa and Lactococcus lactis, with different 28 metabolic features, were used as a comparative experimental model to investigate the antimicrobial 29 target and mechanism of transferrins. In anaerobiosis, P. aeruginosa cells were not susceptible to 30 lactoferrin (hLf) or transferrin (hTf). In aerobiosis, the cells were susceptible but O2-consumptio...

Transcript quantification techniques usually rely on purified mRNAs. We report here a solution-based sandwich hybridization assay for the quantification of mRNAs from Escherichia coli without the need of prior RNA isolation. This assay makes use of four DNA oligonucleotide probes adjacently hybridizing to target RNA in clarified cell extracts. Two helper probes facilitate the hybridization of a...

26 27 Two bacterial species, Pseudomonas aeruginosa and Lactococcus lactis, with different 28 metabolic features, were used as a comparative experimental model to investigate the antimicrobial 29 target and mechanism of transferrins. In anaerobiosis, P. aeruginosa cells were not susceptible to 30 lactoferrin (hLf) or transferrin (hTf). In aerobiosis, the cells were susceptible but O2-consumptio...

The GC-content is very variable in different genome regions and species but although many hypothesis we still do not know the reason why. Here we show that a relationship exists with the mutation rate, in particular we noticed a new recurrence in the amino acids coding table. Moreover we analyze recombination frequency taking into account Single Nucleotide Polymorphisms hourglass distribution. ...

1. The duration of maximal activity and the intensity of anaerobic metabolismwere measured in lowland Sceloporus occidentalis at low (200 ft) and high (10,150 ft) elevations and compared to those of resident S. occidentalis and S. graciosus at high altitude. 2. The duration of maximal activity is independent of altitude: all groups of S. occidentalis had the same stamina, regardless of the alti...

PURPOSE Previous studies have demonstrated that babies are at higher risk for mutans streptococci (ms) colonization if their mothers have dense salivary ms reservoirs relative to babies who have mothers with negligible salivary reservoirs. This communication provides data that identifies another potential risk factor (use of a nursing bottle at bedtime and/or naptime that contains a substrate o...