S1 nuclease hydrolysis and hydroxyapatite chromatography were used to study the effect of the alkylating antibiotic, streptozotocin, on the secondary structure of DNA. Native calf thymus DNA was alkylated in vitro with increasing concentrations of streptozotocin and subjected to S1 nuclease hydrolysis. An increasing degree of DNA degradation was seen, suggesting a destabilization of the seconda...

Newly laid eggs of many insect species store maternal ecdysteroids as physiologically inactive phosphoric esters. In the silkworm Bombyx mori, we previously reported the presence of a specific enzyme, called ecdysteroid-phosphate phosphatase (EPPase), which catalyzes the dephosphorylation of ecdysteroid-phosphates to increase the amount of free ecdysteroids during early embryonic development. I...

Long-chain polyprenols and polyprenyl-phosphates are ubiquitous and essential components of cellular membranes throughout all domains of life. Polyprenyl-phosphates, which include undecaprenyl-phosphate in bacteria and the dolichyl-phosphates in archaea and eukaryotes, serve as specific membrane-bound carriers in glycan biosynthetic pathways responsible for the production of cellular structures...

Objective The aim of this study was to determine the effect of revival soymilk consumption and moderate exercise on vasomotor symptoms, total antioxidant capacity (TAC) and alkaline phosphates activity in postmenopausal women. Materials and Methods In this study, daily for 3 months, 37 postmenopausal women (mean age = 52.2± 4.6 years) were randomly assigned to soymilk (n=15), exercise +soymilk ...

In our studies on perosis in chicks, it was found desirable to be able to determine small amounts of manganese of the order of 0.1 to 10 micrograms. Such a method was necessary for the determination of manganese in bones, blood, and similar materials. The formaldoxime method of Sideris (1) was found unsuitable, as the determination is carried out in alkaline solution. Under these conditions pho...

BACKGROUND Hereditary hemorrhagic telangiectasia (HHT), also known as Osler-Weber-Rendu syndrome, is a rare autosomal dominant genetic vascular dysplasia. OBJECTIVES The aim is to characterize the liver involvement of HHT by multi-detector helical computed tomography. PATIENTS AND METHODS Through the use of multi-detector helical computed tomography, scan data derived from 12 cases of HHT w...

The phosphorus (P) removal of five combinations of dual filters consisting of blast furnace slag (BFS), argon oxygen decarburisation slag (AOD) and electric arc furnace slag (EAF) was evaluated in column experiments with domestic waste water. The columns were fed with waste water for 24 days. The column with only EAF had the best P removal performance (above 93% throughout the experiment). The ...

VLAHOVA-VANGELOVA, D. and S. DRAGOEV, 2014. Marination: effect on meat safety and human health. A review. Bulg. J. Agric. Sci., 20: 503-509 Marinating is commonly used method which involves injection, tumbling or immersion to disperse in the muscle tissue solutions contained water, salt and other ingredients. There are several types of marinating according to added solution ingredients. While a...

Life in oligotrophic environments necessitates quick adaptive responses to a sudden lack of nutrients. Secretion of specific degradative enzymes into the extracellular medium is a means to mobilize the required nutrient from nearby sources. The aquatic bacterium Caulobacter crescentus must often face changes in its environment such as phosphate limitation. Evidence reported in this paper indica...