Alkaline phosphatases may be contributed to the blood by a number of organs; thus the serum alkaline phosphatase may consist of a mixture of phosphatase isoenzymes. Whether or not these isoenzymes differ other than in their tissues of origin has long been debated, some evidence indicating that they are identical, some that they are different. Attempts to characterize alkaline phosphatase by a s...

In this study, multifunctional alkaline pulping was suggested to produce different pulp grades at one mill site, ranging from high-yield paper pulp to high-purity dissolving pulp. In all process modifications, sulfur was successfully replaced by anthraquinone. Autohydrolysis and alkaline pre-extraction were applied to Eucalyptus globulus wood chips, followed by kraft and soda-AQ pulping. Alkali...

Semi-alkaline proteinases having optimum pH between 8 and 9 of Streptomyces origin. have been reported as products of Streptomyces erythreus,3)Streptomyces fradiae4)and Streptomyces madurae.5)The proteolytic activity of all of the above semi-alkaline proteinases can be inactivated by addition of diisopropylfluorophosphate.We have recently isolated a new semi-alkaline proteinase from the culture...

Most protein-based affinity chromatography media are very sensitive towards alkaline treatment, which is a preferred method for regeneration and removal of contaminants from the purification devices in industrial applications. In a previous study, we concluded that a simple and straightforward strategy consisting of replacing asparagine residues could improve the stability towards alkaline cond...

Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection-based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (ssDNA) fluorescent probes through an intermediate RNA library. A double-stranded oligonucleotide library is amplified by transcription to crea...

The present study describes the production, purification and characterization of alkaline protease from mutant strain of Bacillus subtilis EMS-8. The enzyme was purified using ammonium sulphate precipitation which gave 2.64 fold purification with 81.5% yield at 70% saturation. The molecular weight of the enzyme was determined using SDS-PAGE and it was found to be 25 KDa. The optimum pH of enzym...

In our previous work we isolated a cathepsin from the intestinal mucosa of pig and described some of its properties 1 . It is known that proteins are species — and organ speci f ic 2 . W e were interested to know whether this specificity could have any effect on some characteristics of mucosal cathepsin. That such effects may by possible is supported by the results of RANGEL and LAPRESLE 3 who ...

Hepatitis B virus (HBV) core (HBc) virus-like particles (VLPs) are one of the most powerful protein engineering tools utilised to expose immunological epitopes and/or cell-targeting signals and for the packaging of genetic material and immune stimulatory sequences. Although HBc VLPs and their numerous derivatives are produced in highly efficient bacterial and yeast expression systems, the exist...

Bacterial amylase from Bacillus subtilis and fungal amylase from Aspergillus niger were potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase and fungal amylase was done to compare the kinetic parameter of both the types of amylases. 30-60% ammonium sulfate precipitation has done which showed 90 % of purification of both the part...

BENCHMARKS dsDNA was removed from the super-natant (lane 4), which was discarded. The bead-bound dsDNA was denatured in alkaline solution (0.1 M NaOH and 1 mM EDTA) at room temperature for 15 min, and the ssDNA without biotin groups was released in the alkaline solution (lane 5). After neutralization, the ssDNA solution was combined with five volumes of Buffer PB from the MiniElute™ PCR Purific...