Abstract Rapid alkalinization factor (RALF) is widespread throughout the plant kingdom and controls many aspects of life. Current studies on regulatory mechanism underlying RALF function mainly focus Arabidopsis, but little known about role in crop plants. Here, we systematically comprehensively analyzed relation between family genes from five important crops those model Arabidopsis thaliana . ...

We used the absorbance spectrum of the pH-sensitive dye dimethylcarboxyfluorescein to monitor intracellular pH (pHi) in the isolated perfused S3 segment of the rabbit proximal tubule, and examined the effect on pHi of switching from a HEPES to a CO2/HCO3- buffer in the lumen and/or the bath (i.e., basolateral solution). Solutions were titrated to pH 7.40 at 37 degrees C. With 10 mM acetate pres...

UNLABELLED The renal medullary thick ascending limb (MTAL) actively reabsorbs ammonium ions. To examine the effects of NH4+ transport on intracellular pH (pHi) and the mechanisms of apical membrane NH4+ transport, MTALs from rats were isolated and perfused in vitro with 25 mM HCO3(-)-buffered solutions (pH 7.4). pHi was monitored using the fluorescent dye BCECF. In the absence of NH4+, the mean...

We compared the effects of endothelin-1 (ET-1) on intracellular pH, intracellular [Ca 2 1 ] i , and cell contraction in hypertrophied adult ventricular myocytes from ascending aortic banded rats and age-matched controls. Intracellular pH (pH i ) was measured in individual myocytes with SNARF-1, and [Ca 2 1 ] i was measured with indo-1, simultaneous with cell motion. Experiments were performed a...

Airway submucosal glands contribute to airway surface liquid (ASL) composition and volume, both important for lung mucociliary clearance. Serous acini generate most of the fluid secreted by glands, but the molecular mechanisms remain poorly characterized. We previously described cholinergic-regulated fluid secretion driven by Ca(2+)-activated Cl(-) secretion in primary murine serous acinar cell...

The effects of anoxia on intracellular pH (pH(i)) were examined in acutely isolated adult rat hippocampal CA1 neurons loaded with the H(+)-sensitive fluorophore, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. During perfusion with HCO/CO(2)- or HEPES-buffered media (pH 7.35) at 37 degrees C, 5- or 10-min anoxic insults were typified by an intracellular acidification on the induction o...

A B S TRACT We used the absorbance spectrum of the pH-sensitive dye dimethylcarboxyfluorescein to moni tor intracellular pH (pHi) in the isolated perfused $3 segment of the rabbit proximal tubule, and examined the effect on pHi of switching from a HEPES to a CO2/HCOf buffer in the lumen and /o r the bath (i.e., basolateral solution). Solutions were ti trated to p H 7.40 at 37°C. With 10 mM acet...

The contribution of Cl-/HCO3- exchange to intracellular pH (pHi) regulation in cultured chick heart cells was evaluated using ion-selective microelectrodes to monitor pHi, Na+ (aiNa), and Cl- (aiCl) activity. In (HCO3- + CO2)-buffered solution steady-state pHi was 7.12. Removing (HCO3- + CO2) buffer caused a SITS (0.1 mM)-sensitive alkalinization and countergradient increase in aiCl along with ...