A new complex of anti-Gram-positive antibiotics was produced by the fermentation of Actinoplanes arizonaensis sp. nov. The antibiotics were recovered from the fermentation broth with Amberlite XAD-7 resin and from the mycelium by acetone lysis. UV, IR, MS and NMR spectral studies characterized these compounds as kalafungin-type antibiotics. They differ from other known members by an unusual oxi...

Ca2+-pump of sarcoplasmic reticulum was partially purified and solubilized with Triton-X100 in the presence of excess phospholipids. Vesicles were reconstituted by removal of Triton with Amberlite XAD-2 and the subsequent freeze-thaw/sonication method (Kasahara, M. and Hinkle, P. C. (1977) J. Biol. Chem., 252: 7384-7390). In the reconstituted system, H+ was ejected during Ca2+ uptake and in the...

This paper describes a method for the removal of acid-soluble lignin from acid-hydrolyzed hemicelluloses extracted from a mixture of northern US hardwood chips, by using Amberlite XAD-4 resin, which was shown to remove 100% of furan derivatives and 90% of acid-soluble lignin. Subsequent fermentation of the resin-treated hydrolyzates gave ethanol yields as high as 97% of theoretical and showed a...

Methods for the separation of adrenaline, noradrenaline, hydroxytyramine, and dihydroxyphenylalanine (dopa) by employing starch columns (2, 3), paper chromatography (4-7), or countercurrent extraction (8) have limited usage either because of the length of time required for the fractionation or because of the small amounts of material that can be separated. A procedure is described below in whic...

A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavele...

That phosphonium-type ionic liquids (ILs) can be used for preparation of novel solid-phase extractants has been shown. The conditions of IL immobilization on the different matrices (polyacrilonitrile, Amberlite XAD-7, hyper cross-linked polystyrene, and multi-walled carbon nanotubes) were determined. The solid-phase extraction of Pt(IV) from 1-2 M HCl and Pu(IV) from 3 M HNO(3) has been investi...

Abstract Promising green leaching technique was used by Humic acid (HA) for removing uranium from Abu Zeneima spent residue environmental safety and cost-effective leaching. The studied is outlet vat process using sulfuric of carbonaceous shale ore material with initial assays 185 ppm, which representing a hazardous waste. overall efficiency assaying 93% humic at curing temperature 70 °C, 13% H...