introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

Dicarbonyls are known to be important precursors of so-called atmospheric brown carbon, significantly affecting aerosol optical properties and radiative forcing. In this systematic study we report the formation of light-absorbing nitrogen containing compounds from simple 1,2-, 1,3-, 1,4-, and 1,5-dicarbonyl + amine reactions. A combination of spectrophotometric and mass spectrometric techniques...

a series of 2-amino pyridine-3-carbinitrile derivatives incorporated coumarin moiety has developed via multicomponent condensation of 3-acetyl-2h-chromen-2-one, arylaldehydes, malononitrile and ammonium acetate utilizing brønsted acidic ionic liquid, (4-sulfobutyl)tris(4-sulfophenyl)phosphonium hydrogen sulfate as catalyst under solvent-free conditions. good yields, short reaction times, straig...

Purified preparations of diphtheria antitoxin have been obtained by digestion of the toxin-antitoxin complex with trypsin, followed by fractional precipitation with ammonium sulfate. The various fractions obtained in this way are all 90 per cent or more precipitated by diphtheria toxin but combine with different quantities of the toxin. The fraction precipitated between 0.33 and 0.5 saturated a...

Use of enhanced-efficiency N fertilizers (EENFs) in row crop agriculture has not been well studied despite increasing interest in these N sources to increase crop yield while decreasing N loss. A field study was conducted in Central Alabama from 2009 to 2011 to compare EENFs to standard N sources in a high-residue conservation cotton production system. Nitrogen fertilizers evaluated were: urea;...