A recent report by Kern et al. (1) described molecular mechanisms of apoptosis in human hepatocellular carcinoma cell lines in response to treatment with the selective cyclooxygenase-2 (COX-2) inhibitor (coxib), celecoxib. In their study, the authors treated Huh7 and HepG2 hepatocellular carcinoma cell lines with up to 100 Amol/L of celecoxib and found that this treatment led to tumor cell deat...

A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a latex-protein-latex complex (immunocomplex) was formed. The complex was separated from the single latex particles using capillary electrophore...

The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we describe a method for the detection of siRNA in extracts prepared from mammalian cells, which is based on liquid hybridization with a 32P-labelled probe followed by a nuclease protection step. The limit of detection of absolute amounts of siRNA...

A new time-resolved fluorescence immunoassay system involving use of the europium chelate of 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid as label is reviewed. This stable chelate by itself is not very fluorescent but, used in multiple labeling strategies, improves the achievable detection limits. By using multiple labeling, streptavidin tailing, and Eu3+ activation, one...

Cyclic voltammetry shows that yeast iso-1-cytochrome c (YCC), chemisorbed on a bare gold electrode via Cys102, exhibits fast, reversible interfacial electron transfer (k(0) = 1.8 x 10(3) s(-1)) and retains its native functionality. Vectorially immobilized YCC relays electrons to yeast cytochrome c peroxidase, and to both cytochrome cd(1) nitrite reductase (NIR) and nitric oxide reductase from P...

by Prof. Larry Davis An item hot off the press is that the Department’s undergraduate programming team, coached by Amol Deshpande, went to Stockholm to compete with squads from all over the world in a programming contest known as the “Battle of the Brains.” Our team placed 20th, and only three other U.S. universities did better or as well – MIT placed 7’th, CMU 12th and Stanford tied with us fo...

Amol Upadhye, Juliana Kwan, Adrian Pope, Katrin Heitmann, 4, 5 Salman Habib, 4, 5 Hal Finkel, and Nicholas Frontiere 7 Department of Physics, University of Wisconsin–Madison, 1150 University Avenue, Madison, WI 53706 Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104 High Energy Physics Division, Argonne National Laboratory, 9700 S. Cass Ave., Lemont, IL 604...

BACKGROUND Previous studies strongly support an association between unemployment and an increased risk of morbidity and mortality. AIMS To assess the impact of company closure on the mortality of redundant employees. METHODS The mortality of a cohort of male ex-employees of the Athens Bus Transportation Company Epixirisi Astikon Siginonion (EAS) was followed from September 1992 to October 1...

seroepidemiological investigation of hepatitis b,c and hiv virus in safe blood donors of babol blood transfusion center   aghajanipoor k.1,2 (md), zandieh t.1(phd)      1 iranian blood transfusion organization-research center   2 amol regional blood transfusion center