We examined the ability of Aeromonas hydrophila to lyse elastin. Eight of 13 strains showed elastolytic activity on agar medium containing elastin and 5 strains did not. In order to examine the involvement of the metalloprotease of A. hydrophila (AMP) in elastolytic activity, we made the amp-deletion mutant strain from an elastolytic strain. The elastolytic activity of the strain decreased with...

Abstract. The last time in Earth's history when high latitudes were warmer than during pre-industrial times was the interglacial period (LIG, 129–116 ka BP). Since LIG is most recent and best documented interglacial, it can provide insights into climate processes a world. However, some key features of are not well constrained, notably oceanic circulation global carbon cycle. Here, we use new da...

Abstract. Although both physical and biological marine changes are required to explain the 100 ppm lower atmospheric pCO2 of Last Glacial Maximum (LGM, ?21 ka) as compared preindustrial (PI) times, their exact contributions debated. Proxies past carbon cycling (such ?13C) document these thus provide constraints for quantifying drivers long-term cycle variability. This modeling study discusses i...

We report that sphingosine and short-chain ceramides activate adenylate cyclase and stimulate intracellular cyclic AMP formation in airway-smooth-muscle (ASM) cells. In each case, there is a conditional requirement for GTP-Gs alpha. Sphingosine utilizes a protein kinase C-dependent pathway to elicit activation of adenylate cyclase, whereas for short-chain ceramides the mechanism remains unident...

The cyclic dinucleotides cyclic 3′,5′-diguanylate (c-di-GMP) and cyclic 3′,5′-diadenylate (c-di-AMP) have emerged as key components of bacterial signal transduction networks. These closely related second messengers follow the classical general principles of nucleotide signaling by integrating diverse signals into regulatory pathways that control cellular responses to changing environments. They...

Magazines like C&EN that are published by professional societies unique and awkward beasts. They were created to provide members of the society with news about their profession itself. Today, thanks internet, reach can extend well beyond those interested readers worldwide. But such magazines don’t fit neatly within own them. Although many consider a magazine main benefit membership, publishing ...

Abstract. We assess the methodology of so-called 14C plateau tuning (PT) technique used to date marine sediment records and determine reservoir ages (MRAs) as recently reviewed by Sarnthein et al. (2020). The main identified problems are linked assumption constant MRA during age plateaus; lack consideration foraminifera abundance changes coupled bioturbation that can create spurious plateaus in...

Researchers have recently questioned the role hydroxylated prolines play in stabilizing the collagen triple helix. To address these issues, we have developed new molecular mechanics parameters for the simulation of peptides containing 4(R)-fluoroproline (Flp), 4(R)-hydroxyproline (Hyp), and 4(R)-aminoproline (Amp). Simulations of peptides based on these parameters can be used to determine the c...

darafsheh and assari in [normal edge-transitive cayley graphs onnon-abelian groups of order 4p, where p is a prime number, sci. china math. {bf 56} (1) (2013) 213$-$219.] classified the connected normal edge transitive and $frac{1}{2}-$arc-transitive cayley graph of groups of order$4p$. in this paper we continue this work by classifying theconnected cayley graph of groups of order $2pq$, $p > q...

A cyclic AMP-binding protein with a native molecular weight calculated to be 82,000 was purified 2,000-fold from Neurospora crassa. The apparent subunit molecular weight was 47,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, suggesting that the native protein exists as a dimer of identically sized subunits. The 8-N3-cyclic [32P]AMP-labeled protein appeared as a doublet upon tw...