OBJECTIVE The objective of this study was to determine the occurrence and antimicrobial profile of AmpC β-lactamase producing bacteria. METHODS The study was conducted at The Children's Hospital and The Institute of Child Health Lahore, Pakistan, during September 2011 to June 2012. A total number of 1,914 blood samples of suspected neonatal septicemia were processed. Isolates were identified ...

Food contamination with extended-spectrum beta-lactamase (ESBL)-producing bacteria is recognized as one of the public health concerns worldwide. Although fish and fishery products at retail in Japan have relied on imported materials, no apparent evidences exist prevalence property genetic transfer ESBL-producing those food specimens. This study aimed to investigate plasmid-mediated antibiotic-r...

Βeta-lactamase production is the most common mechanism of resistance in Gram-negative bacteria[2] which are also the most common etiological agents in UTI. Extended spectrum betalactamases(ESBLs) and AmpC beta-lactamasesare produced by many Enterobacteriaceae. ‘Plasmids’ carrying ESBL and AmpC genes often carry multiple other resistance genes, thus leading to multi drug resistance in clinical i...

OBJECTIVES Widespread use of β-lactam antibiotics could cause resistance to this group of antibiotics in pathogenic bacteria through the production of the enzyme β-lactamases. The aim of this study is to determine the molecular detection of AmpC β-lactamases among clinical Escherichia coli isolated from Ilam hospitals in Ilam, Iran. METHODS One hundred and twelve clinical isolates of E. coli ...

Enterobacteriaceae clinical strains that produce the class A carbapenem-hydrolysing enzyme KPC (Klebsiella pneumoniae carbapenemase) are increasingly reported worldwide, and are already endemic in North and South America, China, Israel and Greece. The accurate detection of KPC enzymes is of utmost importance for containing the global spread of KPC producers. Currently, the detection of putative...

Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into Escherichia coli and expression experiments gave recombinant clones expressing either an AmpC beta-lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing beta-lac...

One hundred and forty-three non-repetitive strains of Pseudomonas aeruginosa were collected in 13 French hospitals in 1997. A decreased susceptibility or resistance to ticarcillin (MIC > 16 mg/L) was found in 61 isolates (43%) and this was attributed to three major mechanisms: (i) overexpression of OprM and hence related efflux components such as MexAB or MexXY (42.6%), (ii) production of acqui...

Strains of the Enterobacteriaceae family producing ESBL and AmpC broad-spectrum beta-lactamases that may survive in the hospital setting potentially cause infection in hospitalized patients due to contaminated objects or health care workers’ hands. Over a period of two months (November-December 2010), a single epidemiological study of microbial contamination of air, surfaces and health care wor...

Despite the fact that selective pressure of antibiotics on wild birds is supposed to be very weak, they are considered potential vectors antimicrobial resistance (AMR). Obligate scavengers such as vultures can present high proportions extended-spectrum cephalosporins (ESC) and multi-drug-resistant (MDR) bacteria, partially due feeding stations provisioned with livestock carcasses from intensive...

Carbapenems are important agents for the therapy of infections due to multidrug-resistant Pseudomonas aeruginosa; the development of carbapenem resistance hampers effective therapeutic options. To assess the mechanisms leading to resistance, 33 clinical isolates with differing degrees of carbapenem susceptibility were analyzed for the expression of the chromosomal beta-lactamase (ampC), the por...