bacillus sp. guf8, isolated from acidic soil samples of a tea farm was identified as bacillus cereus, based on 16s rdna sequencing and standard bacterial identification methods. following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. consequently, thermostability and kinetic parameters of the purified enzyme wer...

The effect of protease inhibitors on the response of gibberellic acid-treated barley aleurone layers to ethylene was examined. In the absence of protease inhibitors, ethylene plus gibberellic acid initially increased the production of amylase activity relative to layers incubated with gibberellic acid alone. Exposure to ethylene plus gibberellic acid for 48 hours or longer, however, led to depr...

Sequencing upstream of the Streptococcus mutans gene for a CcpA gene homolog, regM, revealed an open reading frame, named amy, with homology to genes encoding a-amylases. The deduced amino acid sequence showed a strong similarity (60% amino acid identity) to the intracellular a-amylase of Streptococcus bovis and, in common with this enzyme, lacked a signal sequence. Amylase activity was found o...

Serum amylase activity was measured in 15 normal persons and in 60 liverdisease patients. Impairment of liver was assessed by serum bilirubin and thymol turbidity values. Most of the patients had serum amylase values that were well below the normal limits. Amylase activities were related to the degree of liver dysfunction, and serum amylase decreased as the bilirubin and turbidity values increa...

introduction: tobacco smoke is involved in the pathogenesis of several diseases in different body systems. saliva is the first body fluid that confront inhaled cigarette smoke which is injurious to the oral cavity and is associated with several oral diseases and cancer. the aim of our study was to evaluate the influence of smoking on the activity of alpha-amylase in the saliva of healthy smoker...

Introduction: Tobacco smoke is involved in the pathogenesis of several diseases in different body systems. Saliva is the first body fluid that confront inhaled cigarette smoke which is injurious to the oral cavity and is associated with several oral diseases and cancer. The aim of our study was to evaluate the influence of smoking on the activity of alpha-amylase in the saliva of healthy smoker...

Protein activation of urinary alpha-amylase (EC 3.2.1.1) activity was observed during an evaluation of the Du Pont aca procedure for the determination of urinary alpha-amylase. This activation effect became constant for urinary albumin concentrations exceeding 1.50 g/liter. It is recommended that urinary alpha-amylase be analyzed with sufficient albumin added to maximize this effect. The aca al...

Saliva is an important body fluid which aids in digestion, in providing a first line of defense and has been recently identified as a repertoire of biomarkers. Salivary composition, hormone levels and enzyme activities are greatly influenced by physiology and well being of the individuals. Oral hygiene is generally maintained by the use of Oral dentifrice like toothpaste and mouthwash. These or...

A monoclonalantibody(66C7) was preparedthat specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and th...