The medaka fish α-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant α-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enz...

Various pulses are a good source of β-Amylase and this enzyme could find promising application of hydrolysis of starch. Hydrolysis of starch by β-amylases has couple of food and pharmaceutical applications. In the present work, β-Amylases from a variety of germinating pulses (Vigna radiata, Cicer arietinum (black), Vigna mungo, Glycine max, Cicer arietinum(white) were extracted and characterize...

Salt-tolerant alkaliphilic actinomycetes are difficult to isolate as their natural environments are much more different and hostile than laboratory conditions. Due to these limitations, majority of the microbial population remain uncultivated. During the recent years, development and application of molecular techniques, such as; DGGE, TGGE, RFLP and DNADNA hybridization have proved quite valuab...

The indiscriminate use of wireless technologies, particularly of cell phones, has increased the health risks among living organisms including plants. We investigated the impact of cell phone electromagentic field (EMF) radiations (power density, 8.55 microW cm(-2)) on germination, early growth, proteins and carbohydrate contents, and activities of some enzymes in Vigna radiata. Cell phone EMF r...

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; howe...