Amyloid associated with seven sclerotic and two normal aortic and mitral valves was studied. The sclerotic valve amyloid contained microfibrils with typical random orientation and a fibril width of 9.5-12.5 nm. The amyloid deposits demonstrated permanganate-resistant Congophilia and contained the amino acid tryptophan. Immunofluorescence studies showed P-component in amyloid deposits of 6 of 7 ...

1. Quantitative scintigraphic and turnover studies, utilizing the specific binding affinity of serum amyloid P component for amyloid fibrils, have been developed as a tool for evaluating amyloid deposits in vivo. 2. Serial studies in over 300 patients have shown characteristic, diagnostic tissue distributions of amyloid in different types of amyloidosis. There is generally a poor correlation be...

The polymorphic β-amyloid lesions present in individuals with Alzheimer's disease are collectively known as cerebral β-amyloidosis. Amyloid precursor protein (APP) transgenic mouse models similarly develop β-amyloid depositions that differ in morphology, binding of amyloid conformation-sensitive dyes, and Aβ40/Aβ42 peptide ratio. To determine the nature of such β-amyloid morphotypes, β-amyloid-...

<b><i>Background:</i></b> Cerebral amyloid angiopathy (CAA) is a common cause of cerebrovascular disease in the elderly. There accumulating evidence suggestive transmissibility β-amyloid resulting pathology at younger age. According to Boston criteria, defining CAA patients <55 years requires histological which may hamper diagnosis. We explored role PET diagnosi...

The successful development of amyloid-based biomarkers and tests for Alzheimer's disease (AD) represents an important milestone in AD diagnosis. However, two major limitations remain. Amyloid-based diagnostic provide limited information about the process they are unable to identify individuals with before significant amyloid-beta accumulation brain develops. objective this study is develop a me...

Abstract A key hallmark of Alzheimer’s disease is the extracellular deposition amyloid plaques composed primarily amyloidogenic amyloid-β (Aβ) peptide. The Aβ peptide a product sequential cleavage Amyloid Precursor Protein, first step which gives rise to C-terminal Fragment (C99). Cleavage C99 by γ-secretase activity releases several lengths and Aβ42 isoform in particular has been identified as...

Alzheimer's disease is characterized by the accumulation of amyloid deposits in the brain and the progressive loss of cognitive functions. Although the precise role of amyloid-β in disease progression remains somewhat controversial, many efforts to halt or reverse disease progression have focussed on reducing its synthesis or enhancing its removal. It is believed that brain and peripheral solub...

Human amyloid deposits always contain the normal plasma protein serum amyloid P component (SAP), owing to its avid but reversible binding to all amyloid fibrils, including the amyloid β (Aβ) fibrils in the cerebral parenchyma plaques and cerebrovascular amyloid deposits of Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). SAP promotes amyloid fibril formation in vitro, contributes...

Background: This study investigated the effect of a mixture Gastrodiae elata and Glycyrrhizae uralensis (GGW) on β-amyloid-induced neuronal damage in vitro. Methods: For finding appropriate GGW ratio, we performed MTT assays using ratios 5:5, 6:4, 7:3, 8:2 9:1 SK-N-SH cell SH-SY-5Y cell. Treatment with β-amyloid (10 μM) caused death overexpression acetylcholinesterase (ACHE) cells. treatment in...

OBJECTIVE Impaired amyloid clearance has been proposed to contribute to β-amyloid deposition in sporadic late-onset Alzheimer's disease (AD). Low density lipoprotein receptor-related protein 1 (LRP-1) is involved in the active outward transport of β-amyloid across the blood-brain barrier (BBB). The C667T polymorphism (rs1799986) of the LRP-1 gene has been inconsistently associated with AD in ge...