Elongation by stems of overwintered tubers of Potamogeton pectinatus (L.) is strongly promoted over several days by oxygen-free conditions. Characteristics of the respiration underpinning this unusual response were examined. Anaerobic plants produced ethanol and CO(2) in approximately equimolar amounts, indicating that glycolysis coupled to alcoholic fermentation was the principal CO(2)-produci...

in this paper, startup performance of upflow anaerobic hybrid reactor and upflow anaerobic sludge blanket reactors in anaerobic treatment of distillery spentwash has been studied under identical conditions of operation. various effluent characteristics like ph, electrical conductivity, chemical oxygen demand, biochemical oxygen demand and total solids and other process parameters like biogas p...

Antimicrobial peptides (AMPs) can cause lysis of target bacteria by directly inserting themselves into the lipid bilayer. This killing mechanism confounds the identification of the intracellular targets of AMPs. To circumvent this, we used a shuttle vector containing the inducible expression of a human cathelicidin-related AMP, LL-37, to examine its effect on Escherichia coli TOP10 under aerobi...

Monitoring studies show that urban surface streams in the United States are commonly contaminated with pesticides, and contamination by organophosphates and carbamates is of particular concern because of their aquatic toxicity. The degradation and sorption of four common organophosphate and carbamate insecticides were studied in urban creek sediments from southern California, USA. In sediment, ...

Fermentation enables the production of reduced metabolites, such as the biofuels ethanol and butanol, from fermentable sugars. This work demonstrates a general approach for designing and constructing a production host that uses a heterologous pathway as an obligately fermentative pathway to produce reduced metabolites, specifically, the biofuel isobutanol. Elementary mode analysis was applied t...

The behavior of natural estrogens was examined by separately spiking 17β-estradiol (E2) and estrone (E1) into semicontinuous activated sludge reactors operated under aerobic, anaerobic and sequential anaerobic/aerobic conditions. Under aerobic and anaerobic conditions, E1 was formed from E2. The conversion of E1 to E2 was maximum conversion of E2 to E1 was higher than t E1 to E2. Overall, the d...

An iron-sensitive mutant of E. coli with a Mudl phage insertion in the nrdB gene lacks subunit B2 of the key enzyme of DNA synthesis, ribonucleotide reductase. Nevertheless, these cells are capable of growing in minimal media under anaerobic conditions, indicating a second enzyme or pathway for deoxyribonucleotide synthesis. We here show that ribonucleotide reduction cannot be unambiguously mea...