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Specific cell surface labeling is essential for visualizing the internalization processes of G-protein coupled receptors (GPCRs) and for gaining mechanistic insight of GPCR functions. Here we present a rapid, specific, and versatile labeling scheme for GPCRs at living-cell membrane with the use of a split green fluorescent protein (GFP). Demonstrated with two GPCRs, GPR17 and CysLT2R, we show t...
We propose a new active learning method for classification, which handles label noise without relying on multiple oracles (i.e., crowdsourcing). We propose a strategy that selects (for labeling) instances with a high influence on the learned model. An instance x is said to have a high influence on the model h, if training h on x (with label y = h(x)) would result in a model that greatly disagre...
The less polar fractions of the extract of the aerial parts of Jurinea leptoloba afforded two new elemanolides. The structures were elucidated by high field NMR techniques
Exponential Linear Units (ELUs) are a useful rectifier for constructing deep learning architectures, as they may speed up and otherwise improve learning by virtue of not have vanishing gradients and by having mean activations near zero [1]. However, the ELU activation as parametrized in [1] is not continuously differentiable with respect to its input when the shape parameter α is not equal to 1...
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