A new hydroxycinnamoyl polyamine derivative, N,N',N"-triferuloylspermidine (= (E)-N-(4-aminobutyl) -3,3',3"-tris(4-hydroxy-3-methoxyphenyl)-N,N',N"- (butane-1,4-diyl)tris [prop-2-enamide]) (1) was detected in the H2O/MeOH extract of pollen from Hippeastrum x hortorum. The compound was identified by on-line-coupled high-performance liquid chromatography and atmospheric-pressure chemical-ionizati...

This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and...

The first hyphenation of high performance liquid chromatography (HPLC), electrochemical online oxidation and mass spectrometry (MS) is described. Ferrocenecarboxylic acid esters of various alcohols and phenols have been synthesized, separated by reversed-phase HPLC and oxidized (ionized) coulometrically prior to single quadrupole MS analysis using electrospray ionization (ESI) and atmospheric p...

The utility of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione (NPTAD) as a derivatization reagent in the analysis of 25-hydroxyvitamin D3 [25(OH)D3] using liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry (LC/ECAPCI-MS) was examined. The derivatives of 25(OH)D3 with NPTAD underwent electron capture in the APCI source in the negative-ion mode and pro...

Risperidone belongs to the new generation of neuroleptics. It is a benzisoxazole derivative. This drug is used in the treatment of acute and chronic schizophrenic psychoses. A method of risperidone determination in whole blood was developed in the course of carrying out an expert investigation concerning a female multi-drug intoxication. Liquid chromatography mass spectrometry (LC/MS) with atmo...

A sensitive liquid chromatograph/tandem mass spectrometric technique (LC/MS/MS) was applied to determine aliphatic and aromatic carbonyl compounds in ambient air. Traces of the carbonyl compounds were sampled by passing through a Sep-Pak DNPH-silica cartridge. Their derivatives were thus eluted with acetonitrile, separated by reversed-phase liquid chromatography and determined by quadrupole tan...

A new sample ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was coupled with a commercial ion trap mass spectrometer. This configuration enables the application-specific selection of external atmospheric ionization sources: the electrospray/APCI (commercially available) and AP MALDI (built in-house), which can be readily interchanged within mi...