The occurrence and spread of antibiotic-resistant bacteria (ARB) are pressing public health problems worldwide, and aquatic ecosystems are a recognized reservoir for ARB. We used culture-dependent methods and quantitative molecular techniques to detect and quantify ARB and antibiotic resistance genes (ARGs) in source waters, drinking water treatment plants, and tap water from several cities in ...

Numerous initiatives have been undertaken to circumvent the problem of antibiotic resistance, including the development of new antibiotics, the use of narrow spectrum antibiotics, and the reduction of inappropriate antibiotic use. We propose an alternative but complimentary approach to reduce antibiotic resistant bacteria (ARB) by implementing more stringent technologies for treating municipal ...

Wastewater treatment plants (WWTPs) functioned as the intersection between the human society and nature environment, are receiving increasingly more attention on risk assessment of the acquisition of environmental antibiotic resistance genes (ARGs) by pathogenetic populations during treatment. However, because of the general lack of robust resistome profiling methods, genotype, and resistance p...

Due to the use of antimicrobials in livestock production, residual antimicrobials and antimicrobial resistance genes (ARGs) could enter the environment following the land application of animal wastes and could further contaminate surface and groundwater. The objective of this study was to determine the effect of various manure land application methods on the fate and transport of antimicrobials...

Evolutionary biologists have introduced numerous statistical approaches to explore nonvertical evolution, such as horizontal gene transfer, recombination, and genomic reassortment, through collections of Markov-dependent gene trees. These tree collections allow for inference of nonvertical evolution, but only indirectly, making findings difficult to interpret and models difficult to generalize....

The aim of this study was to investigate selective pressure of antibiotics on antibiotic resistance genes (ARGs) and bacterial communities in manure-polluted aquatic environment. Three treatment groups were set up in freshwater-sediment microcosms: tetracyclines group, sulfonamides group and fluoroquinolones group. Sediment and water samples were collected on day 14 after treatment. Antibiotic ...

Drinking alcohol causes widespread alterations in gene expression that can result in long-term physiological changes. Although many alcohol-responsive genes (ARGs) have been identified, the mechanisms by which alcohol alters transcription are not well understood. To elucidate these mechanisms, we investigated Gabra4, a neuron-specific gene that is rapidly and robustly activated by alcohol (10-6...

• PE-MP contributed to the spread of ARGs and MGEs in sludge AD process. Strong positive correlations between concentration ARGs/MGEs. reshaped structure composition microbial community. Potential host was identified through co-occurrence networks. Structural equation model quantified contribution MGEs. The retention microplastics sewage sludge, a reservoir antibiotic resistance genes (ARGs) mo...

Antibiotic resistance genes (ARGs) are considered to be emerging pollutants related human activities. The rapid development of global urbanization has expanded activities, thereby exacerbating the health risks caused by antibiotic genes. effects urban and rural environments multifarious, which makes source distribution ARGs in environment diversification. Understanding spread is essential for s...

Comprehensive studies on the effects of genetics and fiber diets antibiotic resistance genes (ARGs) remain scarce. In this study, we analyzed profiles ARGs in colonic contents fecal samples Taoyuan, Duroc, Xiangcun pigs (n = 10) fed at different levels. Through macrogenomic analysis, identified a total 850 unique types classified them into 111 drug classes. The abundance partially drug-resistan...