Blood perfusion of grafted tissue constructs is a hindrance to the success stem cell-based therapies by limiting cell survival and regeneration. Implantation pre-vascularized network engineered in vitro has thus emerged as promising strategy for promoting blood supply deep into construct, relying on inosculation with host vasculature. We aimed fabricate mature microvascular networks, displaying...

Murine cytomegalovirus replicated in reproductive tissue of male mice infected with the virus. We examined three strains of mice latently infected by injection at birth with 100 plaque-forming units of the virus. As adults, these mice contained within their testes 4--6 viral genomic equivalents per 100 cells, as tested by hybridization between mouse DNA and cytomegalovirus DNA. Acutely infected...

Porcine thymus grafts support robust murine and human thymopoiesis, generating a diverse T cell repertoire that is deleted of donor and host-reactive cells, achieving specific xenograft tolerance. Positive selection is mediated exclusively by the xenogeneic thymic MHC. Although thymectomized, T cell-depleted normal mice usually remain healthy following xenogeneic thymic transplantation, thymus-...

Athymic B A L B / c ( N u / N u ) mice died at 7-13 days after inoculation ( D A I ) of P lasmodium berghei NK65 , whereas their heterozygous ( N u / + ) l i t termates died at 7-8 D A I . N u d e ( N u / N u ) mice , reconsti tuted w i th 2 x 10 7 splenocytes f r o m unin­ fected heterozygous ( N u / + ) l i t termates at 20 days before parasite inoculation ( D B I ) , died about 2 days earl i...