rendered background IgE levels only. In accordance, sera of OVA mice which permitted mast cell degranulation upon OVA trigger in a specific b-hexosaminidase release assay, whereas sera of OVA-AAVLP mice did not contain anaphylactogenic antibodies. In an in vivo anaphylaxis experiment, upon intravenous OVA challenge OVA-immunized mice presented significant drop of body temperature, whereas AAVLP...

rendered background IgE levels only. In accordance, sera of OVA mice which permitted mast cell degranulation upon OVA trigger in a specific b-hexosaminidase release assay, whereas sera of OVA-AAVLP mice did not contain anaphylactogenic antibodies. In an in vivo anaphylaxis experiment, upon intravenous OVA challenge OVA-immunized mice presented significant drop of body temperature, whereas AAVLP...

Background: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. Objective: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodi...

In the meeting in 1976 the Committee on Biological Standardization noted that the WHO Working Group on the Standardization of the Human Blood Products and Related Substances had expressed the need of reference materials for anti-A,B, anti-C and anti-E blood-typing sera. At the same time it was informed that the stock of the International Standards for anti-A and anti-B blood-typing sera were de...

Immunoelectrophoretic studies on common antigenicities were carried out by using rabbits sera immunized with the Puerto Rican strain of Schistosoma mansoni adult worms or eggs and antigens of several adult Biomphalaria snails and vice versa. As the result, S. mansoni adult worm extracts produced 8 bands both with extracts of Biomphalaria glabrata pigmentation and B. glabrata pigmentado, 3 to 4 ...

Linear B-cell epitopes of the Rhodococcus equi virulence-associated protein (VapA) were mapped using a synthetic peptide bank in this study. The peptides were screened in an enzyme-linked immunosorbent assay (ELISA) with a total of 70 sera from foals with current R. equi disease (51 sera), as well as from foals that had either recovered from R. equi infection 10 months previously (3 sera) or th...

Serum opsonic capacity against B. pertussis was studied by using quantitative chemiluminescence (CL), a method known to have several advantages over conventional methods in evaluating opsonization and phagocytosis. Sera from unvaccinated infants was shown not to contain opsonins against B. pertussis and in unvaccinated infants suffering from whooping cough, no opsonins were detected. In ad...

We developed an in vitro assay showing that Borrelia burgdorferi organisms were killed by serum from patients with Lyme disease. Twenty of 20 Lyme disease serum samples caused B. burgdorferi killing in a range of 36 to 99% compared with the mean number of viable spirochetes when sera from 10 healthy individuals were used. The percentage of killing of B. burgdorferi increased with convalescent s...

Introduction. The main biological raw materials for the production of immunobiological preparations identification Bacillus anthracis are its specific antigens, protective antigen and EA1 protein.
 Purpose. To determine antigenic identity immunodominant proteins different genovariants B. isolated by gel chromatography electrophoresis.
 Materials methods. Culture filtrates isogenic var...

Serum hepatitis B core antigen (HBcAg) is an important marker of hepatitis B virus replication. We describe an easy, sensitive radioimmunoassay for determination of HBcAg in detergent-treated serum pellets containing Dane particles. Components of a commercial kits for anti-core determination are used, and HBcAG is measured by competitive inhibition of binding of 125I-labeled antibodies to HBcAg...