The cellular fatty acid composition of Brucella ovis and Brucella abortus strains was determined by gas-liquid chromatography. Both species were characterized by the presence of fatty acids 16:0, 17:0, 17:0 cyclopropane, 18:0, 18:1, and 19:0 cyclopropane; B. ovis also contained some 15:0. There were differences in the relative proportions of the fatty acids present, and it was possible to diffe...

We researched the spread of Brucella ovis (B. ovis) infection in sheep during 2002 and 2003 in Croatia. A total of 30,635 sheep blood samples were examined using the enzyme-linked immunosorbent assay (ELISA). In 2002, 1014 out of 14,404 examined sheep blood samples (7%) from six counties gave positive reactions while 2060 (14.3%) were found suspicious. In 2003, 638 out of 16,221 examined sheep ...

OBJECTIVE We report a rare case of a 51-year-old woman with ocular and nasal infestation by Oestrus ovis. METHOD Clinical case report and review of current literature regarding nasal and ophthalmomyiasis. RESULTS Myiasis is infestation of the tissues and organs of vertebrates by certain dipteran fly larvae. Oestrus ovis myiasis is endemic in the region from North Africa to South Asia, but f...

Equine piroplasmosis is a tropical parasitic disease caused by several intraerythrocytic members of the genera Babesia and Theileria. These pathogens are transmitted multiple tick species considered as important exotic microorganisms in countries. causes significant economic losses due to abortions, decreased activity even death equines, making surveillance these infectious essential. In northe...

altogether, 132 rodents of 4 species of meriones persicus , cricetulus migratorius, mus muscri~ius and allactaga dater were collected with live traps from different parts of meshkin - shahr district from summer 1373 to autumn 1374. the purpose of this survey was to identify the parasitic fauna of rodents. before dissection, the mammals were measured for biometric purposes. babesia microti , see...

Bovine babesiosis is a tick-transmitted disease caused by different species of Babesia. The white yak is a unique yak breed that lives only in Tianzhu in the Tibetan Autonomous County, Gansu Province, in northwestern China. Previous research using the ELISA method has confirmed that the white yak could become infected with B. bigemina. The objective of this study was the molecular detection and...

BACKGROUND Borrelia burgdorferi, the causative agent of Lyme disease (LD), and Babesia, Bartonella, and Ehrlichia species (spp.) are recognized tick-borne pathogens in humans worldwide. Using serology and molecular testing, the incidence of these pathogens was investigated in symptomatic patients from Australia. METHODS Sera were analyzed by an immunofluorescent antibody assay (IFA) followed ...

Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RF...

In this preliminary study, a novel DNA microarray system was tested for the diagnosis of bovine piroplasmosis and anaplasmosis in comparison with microscopy and PCR assay results. In the Dakahlia Governorate, Egypt, 164 cattle were investigated for the presence of piroplasms and Anaplasma species. All investigated cattle were clinically examined. Blood samples were screened for the presence of ...

Babesia gibsoni and Babesia canis are the etiological agents of canine babesiosis, a protozoal hemolytic disease with global significance. Canine babesiosis has been diagnosed by microscopic identification of intra-erythrocytic trophozoites in blood smear, and by serological testing. Here we developed a quantitative fluorescence resonance energy transfer (FRET)-PCR that amplifies a fragment of ...