We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...

Preparation and crystallization G6-amylase from alkalophilic Bacillus sp.707 was expressed by Bacillus subtilis 207-25. The enzyme was crystallized by the hanging drop vapor diffusion method using the reservoir solution containing 50% (v/v) 2methylpentane-2, 4-diol, 100 mM Tris-HCl (pH 8.5) and 200 mM ammonium phosphate. Calcium chloride and sodium chloride were added to final 1 mM in the drop ...

Bacillus sp. GUF8, isolated from acidic soil samples of a tea farm was identified as Bacillus cereus, based on 16S rDNA sequencing and standard bacterial identification methods. Following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. Consequently, thermostability and kinetic parameters of the purified enzyme wer...

Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 oC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in...

Addendum: Amplification, Sequencing and Cloning of Iranian Native Bacillus subtilis Alpha-amylase Gene in Saccharomyces cerevisiae [published in Jundishapur J Microbiol. 2013;6(8):e7371] Fahimeh Afzal-Javan ; Mohsen Mobini-Dehkordi ; Behnaz Saffar 1,2 1Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, IR Iran 2Research Institute of Biotechnology, University of Sh...

In bacteria, SsrA, a highly conserved RNA molecule, functions in a mechanism meant to rescue stalled ribosomes. In this process, a peptide tag encoded by SsrA is cotranslationally added to truncated polypeptides, thereby targeting these molecules for proteolytic degradation, at least when they stay inside the cell. This study examined the fate of two extracellular proteins that were tagged by t...

background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...