Outer membrane vesicles (OMVs) are secreted by Gram-negative bacteria via outward budding of the outer membrane. It plays an important role in bacterial-host communication and can modulate host immune response. There limited approaches for fast precise evaluation OMVs secretion. In article e312, Chen, Zhao, coworkers reported a light-up fluorescent AIE probe efficient detection high-throughput ...

The outer membrane, an essential organelle of Gram-negative bacteria, is composed of four major components: lipopolysaccharide, phospholipids, beta-barrel proteins, and lipoproteins. The mechanisms underlying the transport of these components to outer membranes are currently under extensive examination. Among them, the sorting of lipoproteins to the outer membrane of Escherichia coli has been c...

The 325-residue outer membrane protein, OmpA, of Escherichia coli, like most other outer membrane proteins with known sequence, contains no long stretch of hydrophobic amino acids. A synthetic oligonucleotide, encoding the sequence Leu-Ala-Leu-Val, was inserted four times between the codons for amino acid residues 153 and 154 and two, three, or four times between the codons for residues 228 and...

Based on Bayesian Networks, methods were created that address protein sequence-based bacterial subcellular location prediction. Distinct predictive algorithms for the eight bacterial subcellular locations were created. Several variant methods were explored. These variations included differences in the number of residues considered within the query sequence - which ranged from the N-terminal 10 ...

Rickettsia prowazeki were disrupted in a French pressure cell and fractionated into soluble (cytoplasm) and envelope fractions. The envelope contained 25% of the cell protein, with the cytoplasm containing 75%. Upon density gradient centrifugation, the envelope fraction separated into a heavy band (1.23 g/cm3) and a lighter band (1.19 g/cm3). The heavy band had a high content of 2-keto-3-deoxyo...

The major permeability barrier in any membrane is the lipid bilayer structure, and its barrier property is inversely correlated with its fluidity. Bacteria cannot make this membrane much less fluid or it will start to interfere with the normal functions of the membrane proteins, so some bacteria have constructed an additional structure that surrounds the cell outside the cytoplasmic membrane. A...

Fowl cholera continues to be of concern to the poultry industry, especially for turkey growers. This disease costs the turkey industry millions of dollars annually. In order to develop improved live attenuated vaccines or subunit vaccines, the outer-membrane proteins of Pasteurella multocida were examined with the use of proteomics. Of the 11 proteins total present in an outer-membrane subfract...

B acterial cytoplasmic membranes consist primarily of amphipathic phospholipids with lesser amounts of glycolipids, providing a degree of protection from deleterious, toxic substances that is lacking in organisms having a single membrane. Almost all bacterial membranes are assembled in bilayers with embedded integral and associated peripheral membrane proteins. In the case of gram-negative bact...

Bacterial autotransporters represent a diverse family of proteins that autonomously translocate across the inner membrane of Gram-negative bacteria via the Sec complex and across the outer bacterial membrane. They often possess exceptionally long N-terminal signal sequences. We analyzed 90 long signal sequences of bacterial autotransporters and members of the two-partner secretion pathway in si...

CONTENTS I N T R O D U C T I O N . COLONIZATION O F THE INTESTINAL TRACT . . T H E GLYCOCONJUGATE CHEMISTRY O F INTESTINAL S U R F A C E S MEMBRANE GLYCOCONJUGATES . . M U C I N GLYCOCONJUGATES . BACTERIAL A D H E S I N S A N D INTESTINAL RECEPTORS . . FIMBRIAL ADHESINS . Type-I fimbriae . Fimbrial serotypes associated with diarrhoea1 disease . Fimbrial adhesins associated with Helicobacter pyl...