Treatment of wastewater containing carbohydrates by Pichia saitoi growing on beet molasses was investigated in a well-mixed continuous tank as an alternative to bulking control.The yeast strain that used in this work was isolated from non-alcoholic beverage industrial wastewater, with a view on TOC removal compared with other strains in previous study.In this research the isolated yeast showed ...

If sodium pyruvate is added to cells of bakers’ yeast suspended in a phosphate buffer of pH 7.0 in the strict absence of oxygen, practically no pyruvate is utilized. After 3 hours in contact with the cells the sodium pyruvate can be almost quantitatively recovered. If, however, oxygen is admiNed to the cells, then both pyruvate and oxygen are utilized at appreciable rates (see Table I). So far ...

The reduction of glutathione by a heat-labile system in liver was discovered by Hopkins and Elliott (1). Later Mann (2) obtained a soluble enzyme preparation from liver which required, for the reduction process, a cofactor and glucose as hydrogen donor. Meldrum and Tarr (3) found that oxidized glutathione was reduced by rat blood and by yeast and demonstrated the function of TPNl as a cofactor ...

In recent years, industrial fermentation researchers have shifted their attention from liquid to solid and semisolid culture conditions. We converted liquid cultures to the semisolid mode by adding high levels of gelatin. Previous studies on liquid cultures have revealed the inhibitory activity of mineral salts, such as NaCl, on the fermentation of sugars by yeasts. We made a kinetic study of t...

The relationship between lipid content and tolerance to freezing at -50 degrees C was studied in Saccharomyces cerevisiae grown under batch or fed-batch mode and various aeration and temperature conditions. A higher free-sterol-to-phospholipid ratio as well as higher free sterol and phospholipid contents correlated with the superior cryoresistance in dough or in water of the fed-batch-grown com...

The specific activities of 15 aminoacyl-tRNA synthetases in Saccharomyces cerevisiae were measured after growth under a variety of conditions that produced a range of cell-doubling times. The specific activity of each synthetase increased as cell-doubling time decreased. Control experiments eliminate the possibility that these results are due to preferential recovery of synthetases, or to the p...

Ultraviolet-inactivated Hemophilus influenzae transforming DNA recovers its activity when mixed with cell-free extracts of bakers' yeast and exposed to visible light. The active agent in the extract is not used up in the reaction, and purification has not separated it into more than one non-dialyzable component. It differs from the agent in Escherichia coli extract, which produces very similar ...

Renewable energy sources are becoming increasingly important in the beverage and food industries. In the brewing industry, a significant percentage of the used raw materials finishes the process as secondary resource or waste. The research on the anaerobic digestion of brewer's yeast has been scarce until recent years. One of the reasons for this is its use as a secondary resource in the food i...

Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding to one-half and one-fourth of the preparative molecular weight of 119,400 determined by equilibrium cen...