Egg yolk is the most commonly used cryoprotective agent for sperm cryopreservation of several species.The objective of this experiment was to compare the effectiveness of egg yolk from four avian species(domestic chicken, duck, turkey and pigeon) on sperm quality following cryopreservation of the ram semen.Ejaculates were collected, using an electroejaculator, from six fertile rams. Samples wer...

The present study aimed to develop a long-term fresh boar semen extender by adding l-cysteine and docosahexaenoic acid (DHA) into Beltsville thawing solution (BTS)-based extender. Semen samples were collected from six boars, diluted at concentration of 3 × 109 spermatozoa/100 ml allocated 10 groups as follows: BTS, Merck III®, Androstar® Plus, ModenaTM, DHA 1.5 mg/ml, 6.25 2.5 mM, 5.0 mM. All e...

During cryopreservation sperm encounter oxidative stress due to higher production of ROS molecules and insufficient natural antioxidant defence system. Therefore, present study was designed identify the effects various glutathione (GSH) concentrations on Indian red jungle fowl (Gallus gallus murghi) quality fertility pre-freezing post-thaw incubation hours. Semen collected from eight cocks qual...

Cryopreservation of goat spermatozoa is a valuable tool for genetic management. Previous studies have shown the frezeeablity of spermatozoa from various goat breeds but also have difficulties with available protocols. Therefore, we want to investigate the effects of centrifugation and different egg yolk ratios (5% and 10%) on spermatological parameters of Norduz goat spermatozoa. A total number...

The present study was conducted to observe the effect of osmolality of glycerolated TEST-yolk glycerol extenders on post-thawing sperm kinematics of ram spermatozoa of the native Malpura breed maintained in a semi-arid tropical environment. Good quality semen obtained from adult rams was pooled, split and diluted to 1,000 million spermatozoa per ml in complete TEST-yolkglycerol extenders of 900...

The cryopreservation of epididymal sperm is important to preserve genetic material from valuable deceased males. This study evaluated the viability of sperm samples from eight stallions under three conditions: (1) collected using an artificial vagina (EJ-0h), (2) recovered from the epididymal cauda immediately after orchiectomy (EP-0h), and (3) recovered from the epididymal cauda after 24h of s...

Abstract The aim of this study was to determine the effects gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli bucks were used at their breeding season, and ejaculates collected by an electroejaculator. Mixed semen divided into following four groups: control (0 mM), low (L; 1 medium (M; 2 high (H; 4 mM) concentration GA. All groups frozen thawed in a water bath for spermatological...

background: spermatozoa preservation is an approach to improve the fertility rate to pass on the valuable genetic material from sire to their offspring. during the last 20 years, reproductive biotechnologists have focused on the approaches that improve spermatozoa cryosurvival. one of the possible mechanisms is supplementation in semen extender. objectives: the aim of this study was to evaluate...

The aim of the present study was to evaluate the effect of sperm reactive oxygen species (ROS) production and DNA changes on male fertility. For that purpose, six rams with significantly different pregnancy rates were used; these were classified as having high fertility, i.e. 59.4% average pregnancy rate, or low fertility, i.e. 23.1% average pregnancy rate. Sperm quality was assessed after a tw...

To investigate the Effect of adding different concentrations green tea extract as an antioxidant on specific physical semen characteristics German Shepherd dogs during various steps frozen processing this study was carried out. A total (of 20) good Ejaculate samples were collected in a plastic bag from aged 3-4 yearsIn locations (dog breeding).To achieve current study's main objectives, two exp...