background and objectives : streptokinase is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction and is commonly prepared from cultures of streptococcus equisimilis strain h46a. the objective of the present study was the production of streptokinase from strain h46a and purification by chemical reduction method. materials and methods: the rate of...

The object of research is a production of antibiotics by fed batch fermentation. An intelligent system with a structure of neural network and a genetic algorithm are used in nonparametric modeling of the fermentation process. The goal is to build a model for the prediction of fermentation eeciency. A priori knowledge of experts, who are controlling the production of antibiotics in industry, is ...

Lactic acid has been shown to have the most promising application in biomaterials as poly(lactic acid). L. rhamnosus ATCC 10863 that produces L-lactic acid was used to perform the fermentation and molasses was used as substrate. A solution containing 27.6 g/L of sucrose (main composition of molasses) and 3.0 g/L of yeast extract was prepared, considering the final volume of 3,571 mL (14.0% (v/v...

Hyaluronic acid (HA) is a linear high molecular weight glycosaminoglycan polymer made up of repeating units of β (14) N-Acetylglucosamine and β (13) D glucuronic acid. Producing large quantities of high molecular weight polymer with very low dispersity of molecular size is seen as a niche in a huge hyaluronic acid world market and microbial derived product could effectively fit into it. This ...

The results of a successful implementation of multivariate analysis on multiple fermentations are shown in the paper. To minimize batch-to-batch variability of an industrial fermentation process, multi-way partial least squares (PLS) was used. Eighteen baseline batches from different fermentors were analyzed. After applying diagnostics tools and contribution charts, three batches were clearly i...

Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase. To unravel the role of autolysis, an autolysin-coding gene, CA_C0554, was disrupted by using ClosTron system to obtain the mutant C. acetobutylicum ly...

Recombinant alkaline polygalacturonate lyase (PGL) production by recombinant Pichia pastoris GS115 was selected as a model to study as a continuous culture strategy for enhancing heterologous protein production based on controlling methanol feeding (CCCM culture) or on dual carbon source feeding (CCCD culture). Using the CCCM process with a dry cell weight of 75 g/L regulated by controlling met...

Kanjika is a traditional Ayurvedic fermentation product prepares using 11 herbal ingredients in an aqueous media without the addition of any sweetening agents. The aim study was to analyse biochemical changes happening throughout process and identify bioactive molecules formed during process. Two batches were prepared. One batch kept undisturbed process, while samples drawn out at regular inter...

The ability of five yeast strains (locally isolated) to ferment xylose to xylitol were screened using a corn cobs hydrolysate. Candida tropicalis proved to be the best producer. The effects of culture conditions, namely initial pH, nitrogen source and yeast extract concentration on xylitol production were evaluated. The conditions for batch production of xylitol, using C. tropicalis immobilized...