Javert, C. T. (1942). Erythroblastosis neonatorum. An obstetricalpathological study of 47 cases. Surgery, Gynecology and Obstetrics, 74, 1. Kamicki, J. (1968). Results and hazards of prenatal transfusion. Journal of Obstetrics and Gynaecology of the British Commonwealth, 75, 1209. Kitchen, W. H. (1970). Birth weight of infants with Rhesus incompatibility. Australian and New Zealand journal of O...

We describe a new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum. A TBG-beta-D-galactosidase complex was prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester. The mean analytical recovery of TBG added to serum was 99%, sample volume and TBG value were linearly related, and values for TBG determined by this method and those determined by radioimmunoas...

Corticosteroid-binding globulin (CBG) is a serine proteinase inhibitor (serpin) family member that transports glucocorticoids in blood and regulates their access to target cells. The 1.9A crystal structure of rat CBG shows that its steroid-binding site resembles the thyroxin-binding site in the related serpin, thyroxin-binding globulin, and mutagenesis studies have confirmed the contributions o...

A simple experiment was performed to ascertain whether proceduresfor estimating the iron-binding capacity (IBC) of blood serum are quantitative. This was accomplishedby addingf3-globulin (obtained by fractionation of human plasma) to various serum specimens. The 3-globulin solutionswere first assayedfor their IBC. Their addition to serum in these recovery experiments provided data for evaluatio...

The stereospecific binding site for propranolol in cardiac membranes was solubilized with 0.25% sodium deoxycholate. Affinity chromatography on norepinephrine-Sepharose yielded a fraction that bound (-)-propranolol with an association constant 360 times that of the (+)-enantiomer. A Scatchard plot indicated a single binding site with a dissociation constant for propran0101 of 11 + 4 nM and a co...

Searching for a binding protein in blood, which may be involved in the specific transport of cardiac glycosides to their receptor sites on the sodium pump, we isolated a cardiac glycoside-binding protein (CGBG) of 26 kDa from the globulin fraction of bovine serum by affinity chromatography and on a ouabain-Sepharose 4B column by a purification factor of 5000. The cardiac glycoside-binding globu...

An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acet...