AsbA catalyses the highly enantioselective desymmetrisation of citric acid via ATP-dependent condensation with spermidine, as well as the condensation of citric acid with several spermidine analogues and the condensation of the citric acid analogue tricarballylic acid with spermidine, suggesting that it may be a useful biocatalyst for asymmetric synthesis.

INTRODUCTION Proteins are the most versatile molecules in biological world meant for several function including structural organization and catalytic capabilities [1]. Enzymes are the biocatalyst with higher precession and accuracy while performing biochemical reactions. The entire biological world has been evolved with diverse enzymes and microbial population shown tremendous potential in ABST...

To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo a...

A new bacterial nitroreductase has been identified and used as a biocatalyst for the controllable reduction of a variety of nitroarenes with an electron-withdrawing group to the corresponding N-arylhydroxylamines under mild reaction conditions with excellent selectivity (>99%). This method therefore represents a green and efficient method for the synthesis of arylhydroxylamines.

BACKGROUND Lipases including the lipase from Burkholderia cepacia are in a main focus in biotechnology research since many years because of their manifold possibilities for application in industrial processes. The application of Burkholderia cepacia lipase for these processes appears complicated because of the need for support by a chaperone, the lipase specific foldase. Purification and recons...

Deuterium spin relaxation was used to examine the motion of enzyme-bound water on subtilisin Carlsberg co-lyophilized with inorganic salts for activation in different organic solvents. Spectral editing was used to ensure that the relaxation times were associated with relatively mobile deuterons, which were contributed almost entirely by D(2)O rather than hydrogen-deuteron exchange on the protei...

Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it promising biocatalyst for custom tailored microbial oils. C. can accumulate up 60 wt.% its biomass as lipids. This species able grow by using compounds substrate, s...

A novel design of a microbial fuel cell is realized by constructing bio-cocatalyst beads immobilized with riboflavin-secreting Escherichia coli and decoupling them from an anodic biocatalyst. A microbial fuel cell loaded with these bio-cocatalyst beads shows significantly enhanced performance without occupying an active electrode surface area.