BACKGROUND Second-generation ethanol (2G-bioethanol) uses lignocellulosic feedstocks for ethanol production. Sugarcane is one among the most suitable crops for biofuel production. Its juice is extracted for sugar production, while sugarcane bagasse, straw, and senescing leaves are considered industrial waste. Senescence is the age-dependent deterioration of plant cells, ultimately leading to ce...

Zymomonas mobilis is a bacterium that can produce ethanol by fermentation. Due to its unique metabolism and efficient ethanol production, Z. mobilis has attracted special interest for biofuel energy applications; an important area of study is the regulation of those specific metabolic pathways. Small RNAs (sRNAs) have been studied as molecules that function as transcriptional regulators in resp...

Bioethanol is considered to be the most optimistic biofuel. Hydrated ethanol with more than 2% (v/v) of water is not completely miscible with gasoline, so complete dehydration of ethanol is required. Distillation alone cannot concentrate ethanol beyond its azeotropic point so various other methods are used to concentrate ethanol to completely anhydrous state in short span of 93-99.80% (w/w) eth...

In a farmdoc daily article last week (June 3, 2015), we analyzed whether the ethanol mandates recently proposed by the EPA for 2015 and 2016 were high enough to provide a “push” for biofuels use beyond the E10 blend wall. The analysis confirmed that the proposed mandates do indeed imply pressure towards higher ethanol blends or non-ethanol biofuel, but this depends on assumptions about growth i...

Biobutanol has been identified as a promising future biofuel. However, generally the extraction and separation of biobutanol from fermentation mixture is costly process. Therefore, idea using acetone-butanol-ethanol (ABE) directly biofuel were proposed to eliminate recovery ABE The feedstocks play an important role in feasibility fuel. Lignocellulosic biomass seen feedstock for production biofu...

With the vast number of government programmes around the world supporting virtually every phase of biofuels, there is a strong commitment towards the development of these fuels. However, our current knowledge base of biofuel production, marketing and environmental impact is filled with uncertainty. To shed light on the uncertainty, especially from a US perspective, this review of the biofuel ec...

Clostridium acetobutylicum naturally produces acetone as well as butanol and ethanol. Since acetone cannot be used as a biofuel, its production needs to be minimized or suppressed by cell or bioreactor engineering. Thus, there have been attempts to disrupt or inactivate the acetone formation pathway. Here we present another approach, namely, converting acetone to isopropanol by metabolic engine...

BACKGROUND The C4 perennial grass Miscanthus giganteus has proved to be a promising bio-energy crop. However, the biomass recalcitrance is a major challenge in biofuel production. Effective pretreatment is necessary for achieving a high efficiency in converting the crop to fermentable sugars, and subsequently biofuels and other valued products. RESULTS Miscanthus lutarioriparious was pretreat...

BACKGROUND Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial fuel ethanol facilities have found that species of Lactobacillus are predominant. Bacteriophage lytic enzymes are peptidoglycan hydrolases that can degrade the Gram positive ce...

In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orang...