BACKGROUND The application of epidemiologic principles to clinical diagnosis has been less developed than in other clinical areas. Knowledge of the main flaws affecting diagnostic laboratory test research is the first step for improving its quality. We assessed the methodologic aspects of articles on laboratory tests. METHODS We included articles that estimated indexes of diagnostic accuracy ...

The micro-Kjeldahl method for nitrogen determination has been widely used in organic chemistry laboratories in recent years. The value of this method, developed through the brilliant researches of PiLcH, BANG, PARNAS and WAGNER, PREGL, and coworkers, has justified its application to industrial and clinical problems. Plant physiologists have not generally adopted quantitative micro-methods for n...

Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands Max Planck Institute for Biophysical Chemistry, Göttingen, Germany Institute for Physical Chemistry, Georg-August University of Göttingen, Göttingen, Germany Departamento de Física de Materiales, Facultad de Químicas UPV/EHU, Apartado 1072, 20018 Donostia-San Sebastián, Spain ...

Background: BCL-2 is the most important anti-apoptotic regulator and Bax is a pro-apoptotic protein. The status of these parameters or the ration of BCL-2 to BAX is important in malignant cell fate as well as normal cells. Methods: Sixty-two ALL patients and 62 healthy sex-and age-matched controls were studied. After genotyping, the promoter region of the BAX and BCL-2 genes by RFLP-PCR meth...

Two Divisions of the International Union of Pure and Applied Chemistry (IUPAC), namely Physical Chemistry (Commission I.7 on Biophysical Chemistry, formerly Steering Committee on Biophysical Chemistry) and Analytical Chemistry (Commission V.5 on Electroanalytical Chemistry), have prepared recommendations on the de®nition, classi®cation and nomenclature related to electrochemical biosensors; the...

1. Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA 2. Department of Biophysical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany 3. Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, 70569 Stuttgart, Germany 4. Department of Biomedical Engineering, Yale University, New Haven, CT 06520, USA 5. Integrate...

Reported here is a laboratory in vitro evolution (LIVE) experiment based on an artificially expanded genetic information system (AEGIS). This experiment delivers the first example of an AEGIS aptamer that binds to an isolated protein target, the first whose structural contact with its target has been outlined and the first to inhibit biologically important activities of its target, the protecti...

The human myeloid leukemia cell differentiation protein (hMcl-1) is an anti-apoptotic multi-partner protein, belonging to the B-cell lymphoma-2 (Bcl-2) family of proteins. Studies have linked hMcl-1 alleviated expression with resistance hemopoietic chemotherapeutics, which makes it a key drug target in blood cancers. However, most developed small- medium-sized inhibitors typical off-target acti...