Bisulfite sequencing is a valuable tool for mapping the position of 5-methylcytosine in the genome at single base resolution. However, the associated chemical treatment causes strand scission, which depletes the number of sequenceable DNA fragments in a library and thus necessitates PCR amplification. The AT-rich nature of the library generated from bisulfite treatment adversely affects this am...

Whole-Genome Bisulfite Sequencing (WGBS) and genome-wide Reduced Representation Bisulfite Sequencing (RRBS) are widely used to study DNA methylation. However, data analysis is complicated, lengthy, and hampered by a lack of seamless analytical pipelines. To address these issues, we developed a convenient, stable, and efficient web service called Web Service for Bisulfite Sequencing Data Analysi...

Epigenetic changes of DNA, including methylation, have long been recognized as key indicators various diseases, aging, cancer, and neurological disorders. Biomarker discoveries based on distinct methylation patterns for both hypermethylation hypomethylation lead the way in discovery novel diagnosis treatment targets. Many different approaches are present to detect level whole genome (whole bisu...