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The aim of the present study was to explore the roles of OX40/OX40 ligand (OX40L) signaling and OX40+ T cells in ovalbumin (OVA)‑induced mouse asthma model. Asthma was induced by OVA exposure and subsequent co‑treatment with OX40L protein, neutralizing anti‑OX40L blocking antibody, OX40+ T cells or PBS. The protein expression levels of interleukin (IL)‑4, IL‑6, IL‑13, IL‑17, tumor necrosis fact...

OBJECTIVES Inducible costimulator (ICOS) and its ligand (ICOSL) regulate T and B cell responses. Glucose-6-phosphate isomerase (G6PI)-induced arthritis requires T and B lymphocytes. It was hypothesised that blocking ICOS/ICOSL interactions ameliorates G6PI-induced arthritis and reduces G6PI-specific B and T lymphocyte responses. METHODS DBA/1 mice were injected with a blocking, non-depleting ...

THE USE OF A LIQUID PHASE BLOCKING ELISA KIT FOR DETECTION OF ANTIBODIES AGAINST FOOT-AND MOUTH DISEASE VIRUS IN COLOMBIA. The objective of this study was to undertake an interlaboratory comparison of a liquid phase blocking ELISA for detection of antibodies to FMD virus. For that purpose sera from 120 vaccinated, 120 infected and 120 FMD negative cattle were tested. All sera were tested in a s...

BACKGROUND The serological diagnosis of avian influenza (AI) can be performed using different methods, yet the haemagglutination inhibition (HI) test is considered the 'gold standard' for AI antibody subtyping. Although alternative diagnostic assays have been developed, in most cases, their accuracy has been evaluated in comparison with HI test results, whose performance for poultry has not bee...

As a result of the studies, method direct solid-phase enzyme immunoassay (ELISA test) was optimized for detecting toxigenic strains Vibrio cholerae O1 and O139 serogroups based on monoclonal polyclonal antitoxic immunoglobulin peroxidase conjugates. The study detected following optimal parameters analysis: sensitization tablet by toxicodermia drugs diluted in phosphate-saline buffer (PBS) pH 7....

An inactivated SVDV antigen is used in current enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to swine vesicular disease virus (SVDV). To develop a noninfectious recombinant alternative, we produced SVDV-like particles (VLPs) morphologically and antigenically resembling authentic SVDV particles by using a dual baculovirus recombinant, which expresses simultaneously ...

Infectious bovine rhinotracheitis (IBR) is a group of the respiratory disease multifaceted pathogens and key cattle, leading to significant economic losses dairy industry globally. The causative agent IBR Bovine herpesvirus type-1 (BoHV-1), which member genus Varicellovirus in Alphaherpesvirinae subfamily, belongs family Herpesviridae, order Herpesvirales. BoHV-1 can be categorized into three s...

Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 microgr...

VALIDATION OF FAO/IAEA/PANAFTOSA ELISA KIT FOR THE DETECTION OF ANTIBODIES AGAINST FOOTAND-MOUTH DISEASE VIRUS IN VENEZUELA. A liquid phase blocking ELISA (LPBE) supplied by FAO/IAEA/PANAFTOSA has been evaluated for the qualitative and quantitative detection of specific antibodies to "O" and "A" serotypes of foot-and-mouth disease (FMD). A total of 240 bovine sera were analyzed. 120 sera from n...