Peripheral blood mononuclear cells were fractionated according to the expression of a variety of surface markers, and the fractions obtained were tested for erythroid burst-forming unit (BFU-E) colony formation. BFU-Es were detected in the HLA-DR+ non-T cell fraction, but gave rise to optimum colony numbers only in the presence of a nonadherent, relatively radioresistant cell. This accessory ce...

Cord blood has gradually become an important source for hematopoietic stem cell transplantation (HSCT) in the human, particularly in pediatric patients. Adoptive cellular immunotherapy of patients with hematologic malignancies after umbilical cord blood transplant is crucial. Cytokine-induced killer (CIK) cells derived from cord blood are a new type of antitumor immune effector cells in tumor p...

An immunological characterization of leukemias and lymphomas was made in the rat by using a panel of membrane markers in combination with morphological analysis. In the present study, five antigen markers and three surface markers were used for the characterization of 20 rat leukemias and lymphomas, and it was indicated that they could be divided into at least six groups. Of the lymphomas studi...

ndothelial progenitor cells (EPCs) are immature cells that have the capacity to proliferate, migrate, and differentiate into endothelial lineage cells. Embryological research has clarified that embryonic hematopoietic stem cells (HSCs) and EPCs are derived from a common precursor (ie, hemangioblast) and share many surface marker antigens such as Flk-1 (VEGFR2), Tie-2, c-Kit, Sca-1, AC133 (CD133...

Methods Patients of cockroach allergy and healthy controls were selected following American Thoracic Society guidelines. DCs were differentiated from peripheral blood monocytes of patient and control subjects and stimulated with Per a 10. Cells were evaluated for surface markers by flow cytometry and cytokines detected in culture supernatants by ELISA. Autologous DC-T cell cocultures were done ...

A study was done to determine if the differentiation and activation phenotype of T cells in synovial fluid (SF) from patients with juvenile idiopathic arthritis (JIA) is associated with T-cell proliferation in situ. Mononuclear cells were isolated from 44 paired samples of peripheral blood and SF. Differentiation and activation markers were determined on CD4 and CD8 T cells by flow cytometry. C...